National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The employ of in vitro systems allows the dissection of complex interaction between plants and microorganisms, by the analysis of stress factors involved in the plant’s response. Sterile cell cultures are reproducible during long periods and help to the measurement of physiological activities. Plant growth promoting rhizobacteria (PGPR) are a group of microorganisms that are involved in plants’ growth promotion. This study evaluated the effect of particular phytohormones and a plant growth promoting rhizobacteria on the growth and viability of Epithelantha micromeris callus culture. E. micromeris callus growth without the inoculant, diminished in Murashige-Skoog (MS) + naphthalene acetic acid (NAA) medium (34.78%) and MS + indole acetic acid (IAA) (26.08%) experiments. In inoculated callus (B) there was a little promoting effect in: MS+NAA+B (17.39%), MS-VITAMINS+B (30.43%); MS+B (47.82%) experiments, and particularly case was MS+IAA+B experiment, where callus gain 30.43% biomass above the control values obtained. In this work, the presence of some particular auxins and their concentration on non-inoculated and inoculated Epithelantha micromeris callus with a plant growth promoting rhizobacteria, showed a diminished response and even there wasn’t a notably cell dead and the effect of the suggested bioauxins were not evident, the gain of callus fresh biomass was present.
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