International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:4, April, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 32-38

In-Vivo Evaluation of Competitive Parasitic Ability and Rhizosphere Colonisation of Different Trichoderma Isolates
Monika Singh1, O P Sharma2, Someshwar Bhagat2 and Neetu Pandey1
1Department of Biotechnology, Mewar University, Chittorgarh, Rajasthan-312901, India

2ICAR-NCIPM, IARI Pusa, New Delhi-110012, India
*Corresponding author

The rhizosphere competence of Trichoderma isolates was conducted with chickpea, using natural and sterilized soils of Neemrana, Rajasthan. The rhizosphere population of Trichoderma increased at an increasing rate from 15 to 30 DAS and 30 to 45 DAS and thereafter increased but at a decreasing rate and finally declined after 45 DAS, in both soil types. There was appreciable more number of rhizosphere populations of antagonists in rhizosphere soil of test crop observed at 45 DAS than at 15 and 30 DAS. Competitive parasitic ability was conducted in two different soil conditions, viz., natural and steam sterilized soil collected from three states. Under natural soil of Uttar Pradesh, the isolate UP:Bam003 and MP:Kha030 appeared most efficient in their competitive parasitic ability against sclerotia of S. sclerotiorum, whereas the isolate MS:Mar016 was with intermediate effect and the isolate UP:Kus008 were poor competitive colonizer on sclerotia of S. sclerotiorum. Considering the conidial form of inoculum, the isolate UP:Bam003 appeared most efficient colonizer of sclerotia of S. sclerotiorum, whereas UP:Kus008 exhibited as least colonizer and isolate MP:Kha030 and MS:Mar016 showed intermediate colonizing ability of sclerotia. While considering the chlamydospores form of inoculum, the isolate UP:Bam003 was best competitive colonizer of sclerotia of test pathogen showing 91.5% colonizing ability.

Keywords: Rhizosphere colonisation, Competitive parasitic ability, Trichoderma, Sclerotinia sclerotiorum, chickpea (Cicer arietinum L.).

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How to cite this article:

Monika Singh, O.P. Sharma, Someshwar Bhagat  and Neetu Pandey. 2016. In-Vivo Evaluation of Competitive Parasitic Ability and Rhizosphere Colonisation of Different Trichoderma Isolates.Int.J.Curr.Microbiol.App.Sci. 5(4): 32-38. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.