National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Aspergillus spp. is one of the most important fungal spp. In the mycology kingdom and has many important medical, industrial and commercial interesting, it's one of the essential contaminants of food and feed especially under specific circumstances. Aspergillus species was isolated with other fungal species from different samples of feed used by the farmers in different places in the Diyala province such as Fusarium, Aspergillus niger, Aspergillus terrus, Penicillium species and purified in the especial agar media then DNA was successfully isolated from the Aspergillus spp. by using of a commercial kit. PCR was done by using two different primers NS1, C18 and ITS1, ITS4. The first primer sequence didn’t give any result while the second primer revealed the amplification band of all isolated Aspergillus which improve the presence of the a flat oxigenic Aspergillus species in the 10 different feed samples.
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