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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:6, June, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 1903-1913
DOI: https://doi.org/10.20546/ijcmas.2017.606.222


Safety Studies of DpNPV (Diaphania pulverulentalis Nuclearpolyhedrosis Virus) Suspension and its Formulation on Non-Target Organisms in Mulberry Ecosystem
S. Prabhu1*, C.A. Mahalingam2 and S.V. Krishnamoorthy1
1Department of Sericulture, Forest College and Research Institute, Tamil Nadu Agricultural University, Mettupalayam – 641 301, India
2Department of Agricultural Entomology, Tamil Nadu Agricultural University,Coimbatore- 641 003, India
*Corresponding author
Abstract:

Laboratory studies were carried out to explore the potential of Diaphania pulverulentalis (Hampson.) nucleopolyhedrosis virus (DpNPV) suspension and its formulations against non-target organisms viz., Trichogramma chilonis Ishii, Chrysoper lacarnea (Stephens), Bombyx mori (L.), Apiscerena indica (Fab.), Apis mellifera (L.) and Apis florea. The per cent parasitization, adult emergence of T. chilonis exposed to DpNPV suspension did not differ significantly with that of DpNPV formulations and control, while, the per cent parasitization and adult emergence were significantly lower in dichlorvos treated eggs. C. carnea exposed to DpNPV suspension showed no adverse effects on per cent hatchability, larval period, per cent pupation, pupal period, adult emergence, adult longevity, total life cycle and grub mortality in comparison with DpNPV formulations and control. Whereas, dichlorvos was found to be hazardous compared to NPV and control. The larval weight of third, fourth and fifth instar of B. mori, per cent larval mortality, pupation, pupal period, adult emergence cocoon weight and shell weight exhibited no significant differences between the virus treated and control indicating the safety of the DpNPV suspension and its formulation. The survival period of bees in virus treatments was on par with control, while the survival period of bees was significantly low (1-2 days only) in dichlorvos. Results indicated no evidence of infection or other pathological manifestations in the tissues of bees.


Keywords: DpNPV, Diaphania pulverulentalis, Nuclear-polyhedrosis virus, Non-target organisms.

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How to cite this article:

Prabhu, S., C.A. Mahalingam and Krishnamoorthy, S.V. 2017. Safety Studies of DpNPV (Diaphania pulverulentalis Nuclearpolyhedrosis Virus) Suspension and its Formulation on Non-Target Organisms in Mulberry EcosystemInt.J.Curr.Microbiol.App.Sci. 6(6): 1903-1913. doi: https://doi.org/10.20546/ijcmas.2017.606.222
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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