National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Beta-lactamase (EC 3.5.2.6) was isolated and purified from two clinical isolates of Staphylococcus sciuri and Klebsiella pneumoniae by several steps included precipitation with ammonium sulphate at 80% saturation, DEAE-Cellulose and gel filtration on Sephadex G-200 column. The characterization of the purified β-lactamase showed that the molecular weight was 30 KDa for S. sciuri β-lactamase, and 28 KDa for purified K. pneumoniae β-lactamase as estimated by sodium dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The purified enzyme from S. sciuri and K. pneumonae has an optimal temperatures of 35°C and 40°C, respectively. The enzyme from S. sciuri was more stable than that of K. pneumoniae. The optimal pH value were 7.0 and 6.0 from S. sciuri and K. pneumoniae, respectively. The best concentrations of penicillin G were 400 μg ml- and 500 μg ml-1 for the enzyme from S. sciuri and K. pneumoniae. The increase in the enzyme concentration resulted in continuous increase in its activity from both bacteria.
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