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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:6, June, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 595-603
DOI: https://doi.org/10.20546/ijcmas.2017.606.070


Induction of Enzyme Activities in Tuberose Plants Treated with Antagonists and Organic Fungicide under Artificial Inoculation of Sclerotium rolfsii Sacc.
G. Ragavi1, M.L. Mini2*, I. Yesuraja3 and K. Sethuraman4
1Department of Plant Pathology,
2Department of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai-625104, Tamil Nadu, India
3Horticulture Research Station, Thadiyankudisai, Dindigul - 624 212, Tamil Nadu, India
4Maize Research Station, Vagarai, Dindigul - 624 613, Tamil Nadu, India
*Corresponding author
Abstract:

A pot experiment was conducted to study the antioxidant responses of tuberose treated with biocontrol agents and organic fungicide in combating wilt disease caused by Sclerotium rolfsii Sacc. The antagonists, Trichoderma viride4 (Tv4), Bacillus subtilis1 (Bs1) and Pseudomonas fluoresence3 (Pf3) were used alone and in combinations. Mahua cake was used as the source of organic fungicide. The treatment with the commonly used chemical fungicide, hexaconazole was taken as check. Among the fifteen treatments, soil application of Tv4 @2.5 kg ha-1 + Pf3 @2.5 kg ha-1 + Bs1 @ 2.5 kg ha-1 + Mahua cake @ 250 kg ha-1 recorded the highest enzymatic activities of peroxidase, polyphenol oxidase and phenylalanine ammonia lyase on the fourth day after challenge inoculation with Sclerotium rolfsii Sacc. This treatment also recorded the highest phenol content (1.179 µg g-1 FW) on the fourth day after challenge inoculation with S. rolfsii. This treatment recorded the lowest wilt incidence (7.56 %) which indicated 90.41 percent disease reduction over control.


Keywords: Antagonists, Peroxidase, Polyphenol oxidase, Sclerotium rolfsii, Tuberose.

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How to cite this article:

Ragavi, G., M.L. Mini, I. Yesuraja and Sethuraman, K. 2017. Induction of Enzyme Activities in Tuberose Plants Treated with Antagonists and Organic Fungicide under Artificial Inoculation of Sclerotium rolfsii Sacc..Int.J.Curr.Microbiol.App.Sci. 6(6): 595-603. doi: https://doi.org/10.20546/ijcmas.2017.606.070
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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