National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bunchy top disease caused by the banana bunchy top virus (BBTV) is a serious disease in hill banana. Detection of the BBTV infection in the planting material could help in the effective management of the disease. Primers were designed for the PCR amplification of BBTV coat protein (CP). In the present study, to confirm the virus infection and to quantify the viral load in the plants, samples from infected and healthy banana were subjected to PCR and southern blot hybridization. There was no distinguishable difference in viral load with respect to age of the plants was observed. The BBTV capsid protein (CP) gene located on component 3 was cloned in an expression vector pET28a (+). Expression of the CP in E. coli BL21 cells was induced by adding isopropyl-3-D-1-thiogalactoside (IPTG) to a final concentration of 1 mm. The expressed CP which migrated as a protein of approximately 19.5 kDa in Sodium Dodecyl Sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE) was identified by its molecular weight. Hence it is concluded that the CP gene of BBTV was cloned and expressed in E. coli can be used for antibody rising or adding an affinity purification tag will enable us to produce pure monoclonal antibody in future.
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