National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Recently evolved technique, Clustered Regularly Interspaced Palindromic Repeats (CRISPR)-CRISPR-associated (Cas9) has added new armory for the genome editing approaches. This CRISPR/Cas9 pathway of archaeal and bacterial defense mechanism against the invading genomic material utilizes a short guide RNA to direct the endonuclease Cas9 to cut the foreign genetic material and provide resistance against the same. The immunity in archaea and bacteria is developed due to the transcription of cut segment of the exogenous material which has been incorporated in host genome system as memory which is transcribed in the form of guide RNA. So by artificially synthesizing the desired guide RNA, Cas9 can be virtually directed anywhere in the genome to cause DNA double strand breaks (DSBs) and can accomplish the repair or insertion, deletion etc actions to edit genome of the organism in desired directions. The manifestation of this novel technique depends on the presence of PAM (protospacer adjacent motif) sequence which lies downstream to the target site. Hence here we are discussing the concept and use of CRISPR/Cas9 mechanism that can be a very efficient and indispensable tool for genetic manipulation in future.
Citations |
 |
 |
 |
 |
