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Original Research Articles                      Volume : 14, Issue:3, March, 2025

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2025.14(3): 165-171
DOI: https://doi.org/10.20546/ijcmas.2025.1403.019


Isolation of Microorganisms from the Barren Soil of Gorakhpur, Uttarpradesh, India
Kriti Mishra1 and Deepa Srivastava2*
1Department of Microbiology, Deen Dayal Upadhyay Gorakhpur University, Gorakhpur, India 2Department of Botany, Deen Dayal Upadhyay Gorakhpur University, Gorakhpur, India
*Corresponding author
Abstract:

This article clarifies the presented content regarding the isolation of microorganisms from the barren soil and elucidate their presence in soil. This content is focused to isolate microbiota from the soil which have not capacity to yield properly and cannot be suitable for agriculture. The microbial community plays their role in plant growth same as inorganic and organic materials. The purpose is to focus on the idea that microbes are present or absent in soil when it is considered as barren which means lack of productivity. To know the type of biota and to find out their role in soil, isolation is required. To isolate biota, various methods; as serial dilution, media prepration (LB media-Luria Bertani media and PDA media-Potato Dextrose Agar media), streaking and spreading methods, Gram staining, Antibiotic susceptibility test, DNA extraction, gel electrophoresis are performed. Passing through the process of isolation, there is bacterial and fungal colony observed but there are very few numbers of colony and less productive microbes are isolated. Therefore, it can be concluded that barren soil also contains microbes but in less quantity and with low viability.


Keywords: Soil, Barren soil, Soil microflora, Isolation, Identification


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How to cite this article:

Kriti Mishra and Deepa Srivastava 2025. Isolation of Microorganisms from the Barren Soil of Gorakhpur, Uttarpradesh, IndiaInt.J.Curr.Microbiol.App.Sci. 14(3): 165-171. doi: https://doi.org/10.20546/ijcmas.2025.1403.019
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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