National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The inappropriate use of antibiotics since their discovery has been the cause of the appearance of resistant or multi-resistant bacteria both in the environment and in hospitals. Although major organizations such as the WHO have set up several systems to combat bacterial resistance, it remains a major public health problem. In order to assess bacterial resistance in Brazzaville's soils and clinics, strains of Staphylococcus and Enterobacteriaceae were isolated and antibiogram. The results of this study show that a wide variety of potentially pathogenic bacteria can be found in soil. The distribution of species isolated according to their biological origin revealed that the majority of Staphylococcus came from vaginal swabs (75%), followed by urine (15%) and spermatic fluid (10%), while the majority of Enterobacteriaceae came from urine (55%), followed by vaginal swabs (35%) and spermatic fluid (10%). The majority of strains used were resistant to almost all the antibiotic families used for susceptibility testing. Hospital-derived Staphylococcus strains showed very high rates of resistance (55 to 100%) to all antibiotics tested, compared with soil-derived strains. Soil-derived strains also showed high levels of resistance to certain molecules. Enterobacteriaceae strains were resistant to quinolones. Strains from both environments were more resistant to beta-lactam antibiotics (FOX CXM, CEX, IMI, ATM), with the exception of Ertapenem, where the resistance rate was higher in soil strains (55%) and lower in hospital strains (30%). OFX, NA, MXF and NOR showed very high rates, as did other antibiotic molecules (colistin and kanamycin). This work has shown us an increase in resistance in clinical and environmental strains to the most widely used antibiotics. This is a cause for concern in the therapeutic management of patients, and can lead to high costs for prolonged hospitalization, increased morbidity and mortality.
Ahombo Gabriel, Moyen Rachel, Baloki Ngoulou Tarcisse, Kayath Aimé Christian, Ontsira Ngoyi Nina Esther 2019, Genetic Determinism in Community Staphylococcus and Methicillin-Resistant Clinics in Brazzaville, Republic of Congo. International Journal of Innovative Research in Medical Science.04, Issue 07, July 2019. https://doi.org/10.23958/ijirms/vol04-i07/707
Baloki Ngoulou Tarcisse, Ahombo Gabriel, Nguimbi Etienne, Ampa Raoul et Moyen Rachel. (2019). Caractérisation moléculaire et distribution des gènes codant pour la résistance aux macrolides. Lincosamides et Streptogramines B dans les Staphylocoques communautaires et cliniques à Brazzaville, Congo. Afrique Sciences. 15(5), 352-363.
Berge Berg, G., Eberl, L. & Hartmann, A. (2005). The rhizosphere as a reservoir for opportunistic human pathogenic bacteria. Environmental Microbiology 7, 1673-1685. https://doi.org/10.1111/j.1462-2920.2005.00891.x
Boukhatem, L. (2013) Etude de la sensibilite aux antibiotiques des bacilles Gram negatif non fermentant isoles au niveau du service de reanimation du CHU de Tlemcen. UniversiteAboubekrBelkaid Tlemcen, memoire de master. 85 p.
Bouyahya A. Y., Bakri A., Et-Touys A., Talbaoui A., Khouchlaa S., Charfi J., Abrini N. Dakka. (2017). (Résistance aux antibiotiques et mécanismes d’action des huiles essentielles contre les bactéries Phytothérapie. https://doi.org/10.1007/s10298-017-1118-z
CA-SFM (2023) Comite de l’Antibiogramme de la SocietéFrancaise de Microbiologie. https://www.sfm-microbiologie.org/wp-content/uploads/2023/06/CASFM2023_V1.0.pdf
Christensen G.D, Simpson W.A, Younger J.A, Baddour L.M, Barrett F.F, Melton D.M and Beachey E.H. (1985). Adherence of cogulase negative Staphylococi to plastic tissue cultures: a quantitative model for the adherence of staphylococci to medical devices. J.ClinMicrobiol. 22: 996–1006. https://doi.org/10.1128/jcm.22.6.996-1006.1985
Clinical and Laboratory Standards Institute, CLSI (2010) Surveillance for Methicillin- Resistant Staphylococcus aureus : Principales, Practices, and Challenges : A Report. 14 p. https://clsi.org/media/1941/microvastmeetingminutes_2010jan.pdf.
Colinon, C., Deredjian, A., Hien, E., Brothier, E., Bouziri, L., Cournoyer, B., Hartman, A., Henry, S., Jolivet, C. &Ranjard, L. (2013). Detection and enumeration of Pseudomonas aeruginosa soil and manure assessed by an ecfX qPCR assay.J Appl Microbiol. 114(6):1734-49. https://doi.org/10.1111/jam.12189.
Dia M.L., Diongue K., Diop A.,.Ka R, Sonko M.A., Diagne R., Sow A.I., Cisse M.F (2014): Mechanisms of β-lactam resistance in isolated Staphylococcus aureus strains at Dakar (senegal) hospital, Cames Health,(2), pp. 9-12.
Durmaz, B., Durmaz, R. and Sahin, K. (1997) Methicillin-Resistance among Turkish Isolates of Staphylococcus aureus Strains from Nosocomial and Community Infections and Their Resistance Patterns Using Various Antimicrobial Agents. Journal of Hospital Infection, 37, 325-329. https://doi.org/10.1016/S0195-6701(97)90149-3
Fahimeh G., Ghajavand H., Havaei R., Mohammad J.S., Khademi F., Heydari L., Shahin M., Havaei S. A (2016): Distribution of erm genes among Staphylococcus aureus isolates with inducible resistance to clindamycin in Isfahan, Iran, Adv. Biomed Res., (5), pp 62. https://doi.org/10.4103/2277-9175.179184
Freney Jean, Renaud François, Leclercq Roland, Riegel Philippe, Monteil, Henri, Étienne, Jérôme (2007). Précis de bactériologie clinique. 2e édition. Paris Lyon : Éditions ESKA Éditions Alexandre Lacassagne, Print.
Gangoue Léa Gwladys, Nguimbi Etienne, OkambaOndzia Faust René, Banzouzi Mahoukou BricelineP rincesse, Moutali Lingouagou Tantique, Baloki Ngoulou Tarcisse, Onyankouang Isaac Samuel, Moyen Rachel. (2021). Prevalence, Resistance to Quinolones/ Fluoroquinolones of Staphylococcus aureus Strains Isolated in the Hospital and University Center of Brazzaville, Republic of Congo. Advances in Microbiology. 11, 607-615.
https://doi.org/10.4236/aim.2021.1111044.
Kayode K.. Ojo, J.S. Megan, Ulep C.C., Natasha S. C., Zittle J., Henrique L., Bernardo M., Leitao J., Roberts M.C (20206): Effruxmsr (A) Characterized Gene in Streptococcus, Enterococcus, Corynebacterium, and Pseudomonas Isolates, Antimicrob Agents Chemother, 50 (3), pp.1089-1091.
Longtin Jean, Cindy Lalancette, AlejandraIrace-Cima, Christian Lavallée, Anne-Marie Bourgault, M.D. (2017). Cadre normatif pour l’antibiogramme minimal à réaliser par les laboratoires du Québec.14P.
Matute AJ, Hak E, Schurink CAM, Mc-Arthur A, Alonso E, Paniagua M, van Asbeck E, Roskott AM, Froeling F, Rozenberg-Arska M, Hoepelman IM (2004). Resistance of uropathogens in symptomatic urinary tract infections in León, Nicaragua. International Journal of Antimicrobial Agents 23:506-509 https://doi.org/10.1016/j.ijantimicag.2003.10.003
Morabandza Cyr Jonas, Nguimbi Etienne, BalokiNgoulouTarcisse, Moyen Rachel, MoussoundaMilongo Lucien Jolivet, Soloka Mabika Armel Faly, Ngo-Itsouhou Christian, Ahombo Gabriel. (2020). Antibiotic Resistance Profile of Pathogenic Bacteria Isolated from “Mabokés” Smothered Fish in Brazzaville, Congo. Journal of Biosciences and Medicines, 8, 138-148. https://www.scirp.org/journal/jbm.
Moutou T. D. P., Nguimbi E., Giusti-Miller S., Mora P., Kobawila S. C., Miambi E. (2018). Assessment of dominant bacterial strains isolated from Ntobambodi, an indigenous African alkaline fermented food, and their potential enzyme activities. Afr Jornal Microbiology Research, 12(32), 779-787. http://dx.doi.org/10.5897/AJMR2018.8875
Moyen R., Ahombo G., Nguimbi E., Niama R. F., Ontsira N. E., Yala G. C, Obengui, et Louembe D (2012). Résistance aux antibiotiques des souches de Pseudomonas aeruginosa isolées des infections de plaies au Centre Hospitalier et Universitaire de Brazzaville. Sciences et Médecine. 13(2): 98 -101.
Moyen Rachel, Baloki Ngoulou Tarcisse, Nguimbi Etienne, Ahombo Gabriel (2021) Antibiotic Resistance of Enterobacteriaceae isolated from household Wastewater in Brazzaville, Republic of Congo. Advances in Microbiology. 11, 27-36. https://doi.org/10.4236/aim.2021.111003
Moyen Rachel, EmpiloNdjiwaGalekoua Saphia Jemylah, Yala Jean Fabrice, Bokatola Pea Indra Roenate, BalokiNgoulouTarcisse. 2022 Evidence of the transmission of resistance of enterobacteria to the betalactamines between mothers and children in Brazzaville. American Journal of Applied Scientific Research. ISSN: 2471-9722 (Print); ISSN: 2471-9730. 8(2): 30-37. https://doi.org/10.11648/j.ajasr.20220802.12
Mpelle Fils Landry, OntsiraNgoyi Esther Nina, Kayath Christian Aimé, Nguimbi Etienne, Moyen Rachel and Kobawila Simon Charles. (2019). First report of the types TEM, CTX-M, SHV and OXA-48 of beta-lactamases in Escherichia coli, from Brazzaville, Congo. African Journal of Microbiology Research. Vol. 13(8), pp. 158-167. https://doi.org/10.5897/AJMR2018.9042.
Mukubwa, G. K., Lukusa, F. N., Kavulikirwa, O. K., Liesse, J. I., Tshilolo, L. M., et Memvanga, P. B. (2023) Profils de résistance des isolats urinaires d'Escherichia coli et de Klebsiella pneumoniae aux antibiotiques couramment prescrits pour le traitement des infections des voies urinaires au Centre Hospitalier Monkole, Kinshasa, République Démocratique du Congo:24. 1. https://dx.doi.org/10.4314/ajcem.v24i1.7.
Mukubwa, G. K., Lukusa, F. N., Kavulikirwa, O. K., Liesse, J. I., Tshilolo, L. M., et Memvanga, P. B. (2023) Profils de résistance des isolats urinaires d'Escherichia coli et de Klebsiella pneumoniae aux antibiotiques couramment prescrits pour le traitement des infections des voies urinaires au Centre Hospitalier Monkole, Kinshasa, République Démocratique du Congo: https://dx.doi.org/10.4314/ajcem.v24i1.7
Munoz-Price LS, Poirel L, Bonomo RA, Schwaber MJ, Daikos GL, Cormican M, Cornaglia G, Garau J, Gniadkowski M, Hayden MK, Kumarasamy K (2013). Clinical epidemiology of the global expansion of Klebsiella pneumoniae carbapenemases. The Lancet Infectious Diseases 13(9):785-796. https://doi.org/10.1016/s1473-3099(13)70190-7
Ndelilionel (2009. Etude des prescriptions des antibiotiques officinal. Cas de la pharmacie LAFIA en commune IV de Bamako. 96 P.
https://www.bibliosante.ml/handle/123456789/7091
Nedjai S, Barguigua A, Djahmi N, Jamali L, Zerouali K, Dekhil M, Timinouni M (2012). Prevalence and characterization of extended spectrum β-lactamases in Klebsiella-Enterobacter-Serratia group bacteria, in Algeria. Infectious Diseases Medicine 42(1): 20–29. Not cited https://doi.org/10.1016/j.medmal.2011.10.001
Nguimbi E., Morabandza C. J., Vouidibio M. A. B., Mbou M. H. B., Miakassissa S. N., Soloka M. F. A. (2020). Microbial Biodiversity of a Traditional Food Made from Squash Seeds "NTETE" Consumed in Brazzaville, Republic of Congo. International Journal of Microbiology and Biotechnology. Vol. 5, No. 3, 2020, pp. 83-92. https://doi.org/10.11648/j.ijmb.20200503.12.
Nordmann P, Carrer A (2008). Carapenemases of enterobacteria Carbapenemases of enterobacteria Carbapenemases in enterobacteriaceae. Pediatrics Archives 17:S154-S162.
Nouri. M, Ziadi.C. (2015). Etude bactériologique et résistance aux antibiotiques de Klebsiella Pneumonie Génétique moléculaire, Université des frères mentouri Constentine.69P.
Nouria L, Hafida H, Fréderic R, Richard B, Djamel-Eddine A (2016). Prevalence and molecular typing of extended-spectrum –lactamases in Escherichia coli, Enterobacter cloacae and Citrobacter freundii isolates from Laghouat Hospital, Algeria. African Journal of Microbiology Research 10(35):1430-1438. http://dx.doi.org/10.5897/AJMR2016.8263
Ouedraogo AS, Jean Pierre H, Banuls AL, Ouedraogo R, Godreuil S (2017). Emergence and spread of antibiotic resistance in West Africa : contributing factors and threat assessment. Medicine and Health Tropicales 27: 147-154. https://doi.org/10.1684/mst.2017.0678
OuLd Baba Ali R, Taibi K (2019).Etude de l'antibiorésistance de souches d'enterobacteries productrices de B-lactamases a spectre étendu (BLSE) isolée à l'hôpital de Boufarik. http://di.univ-blida.dz:8080/xmlui/handle/123456789/1381
Paterson DL (2000). Recommendation for treatment of severe infections caused by Enterobacteriaceae producing extendedspectrum beta-lactamases (ESBLs). Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases 6(9): 460–463. https://doi.org/10.1046/j.1469-0691.2000.00107.x
Paterson DL, Bonomo RA (2005). Extended-spectrum β-lactamases: a clinical update. Clinical Microbiology Reviews 18(4):657-686. Philippon A, Labia R, Jacoby G (1989). Extended-spectrum betalactamases. Antimicrobial Agents and Chemotherapy 33(8): 1131–1136.
Prats, G., Mirelis, B., Llovet, T., Munoz, C., Miro, E. and Navarro, F. (2000) Antibiotic Resistance Trends in Enteropathogenic Bacteria Isolated in 1985-1987 and 1995-1998 in Barcelona. Antimicrobial Agents and Chemotherapy, 44, 1140-1145. https://doi.org/10.1128/AAC.44.5.1140-1145.2000.
Prescott, Harley, Klein, Wiley, Sherwood et Woolverton. (2010). Parties VIII. Ecologie et symbiose. In : Microbiologie. Traduction de la 7ème édition américaine. Editions DeBoeck Université Bruxelles. pp: 643-739.
Rebiahi SA, Abdelouahid DE, Rahmoun M, Abdelali S,Azzaoui H. (2011) Emergence de Staphylococcus aureus résistant à la vancomycine identifiée au CHU de Tlemcen (Nord-Ouest algérien): 22033230 https://doi.org/10.1016/j.medmal.09.010.
Sekhsokh Y, Chadli M, El Hamzaoui SA. (2008). Fréquence de Sensibilité aux antibiotiques des bactéries isolées dans les urines. Médecine et Maladies Infectieuses. 38, 6,324-327. https://doi.org/10.1016/j.medmal.2008.02.003.
Sire JM, Nabeth P, Perrier-Gros-Claude JD, Bahsoun I, Siby T, Macondo EA, Garin B (2007). Antimicrobial resistance in outpatient Escherichia coli urinary isolates in Dakar, Senegal. Journal of Infection in Developing Countries 1(3):263-268.
Soumaila Garba A., (2012). Caractérisation phénotypique et génétique des Escherichia coli isolés des cas de colibacilloses aviaires au Sénégal. Thèse doctorat en médicine vétérinaire. Sénégal.107P.
Storberg V (2014). ESBL-producing Enterobacteriaceae in Africa – a non-systematic literature review of research published 2008–2012. Infection Ecology and Epidemiology 4(1):20342. https://doi.org/10.3402/iee.v4.20342
Tansarli GS, Poulikakos P, Kapaskelis A, Falagas ME (2014). Proportion of extended-spectrum β-lactamase (ESBL)-producing isolates among enterobacteriaceae in Africa: Evaluation of the evidence-systematic review. Journal of Antimicrobial Chemotherapy 69(5):1177-1184. https://doi.org/10.1093/jac/dkt500
Touati A, Medboua C, Touati D, Denine R, Brasme L (2012). CTX-M- 15-producing Enterobacteriaceae isolates causing bloodstream infections at the Beni-Messous hospital in Algiers (Algeria). International Research Journal of Microbiology 3(5):18-185.
ZohounA., Dao I., Karfo R., Essayagh T., Sekhsokh Y., Bousta M., El Hamzaoui S. (2012). Méningite nosocomiale postopératoire à Acinetobacter baumannii multirésistant en neurochirurgie : à propos d’un cas : 60. 2
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