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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 14, Issue:3, March, 2025

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2025.14(3): 112-117
DOI: https://doi.org/10.20546/ijcmas.2025.1403.015


Identification and isolation of E. coli Bacteria from RO Water in the Center of Karbala and its Surrounding Areas
Alaa Yaqoob Rahi1* and Z. Abdul Alamir Mezher2
1Department of Science, College of Basic Education, University of Sumer, Iraq
2Department of biology, college of education for pure science, university of Karbala, Karbala, Iraq
*Corresponding author
Abstract:

The aim of this research was to find out if the water (Ro) in the majority of Iraqi houses contains E. coli bacteria because of the prevalence of diarrhea and other illnesses among the populace. The goal was accomplished by. The study samples were collected from R. O water filling stations in Karbala, including 40 samples from Karbala Center, 20 samples from Tuwairij District, 20 samples from Al-Hussainiya District, and 20 samples from Ain Al-Tamr District for the purpose of investigating the E. coli bacteria contaminating these waters, where microscopic and cultural examinations and biochemical tests were used. The results of these tests showed that 3 samples of R.O water out of 100 samples, with an isolation rate of 4.5%, gave positive results through their growth on selective and differential media and their fermentation of lactose sugar. On the other hand, the research results showed that the highest percentage of bacteria isolation was from R.O water filling stations in Ain Al-Tamr district with a percentage of 2.5) 2.2%) and the lowest percentage was from R.O water filling stations in Al-Hussainiya district with an isolation percentage of 1.7) 1.5%), while R.O water filling stations in the center of the governorate of Karbala and Twerij district did not record the least contamination with coliform bacteria (0.5 %). Through the research, the results showed that R. O water in some areas is contaminated with E. coli bacteria, which may be the source of human waste, which indicates the inefficiency of these stations.


Keywords: Bacteria, E. coli, R.O water, enterobacteriaceae family


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How to cite this article:

Alaa Yaqoob Rahi and Abdul Alamir Mezher, Z. 2025. Identification and isolation of E. coli Bacteria from RO Water in the Center of Karbala and its Surrounding Areas.Int.J.Curr.Microbiol.App.Sci. 14(3): 112-117. doi: https://doi.org/10.20546/ijcmas.2025.1403.015
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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