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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 14, Issue:3, March, 2025

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2025.14(3): 98-104
DOI: https://doi.org/10.20546/ijcmas.2025.1403.013


Effect of Biofilm Formation on Antibiotic Resistance in Gram Positive Organisms Isolated from Central Line Associated and Central Line Related Blood Stream Infection at a Tertiary Care Hospital
Dharmendra Singh1 and Anita E. Chand2*
1Ph.D. Scholar, Department of Microbiology, Government Medical College, Kota, Rajasthan, India
2Senior Professor, Department of Microbiology, Government Medical College, Kota, Rajasthan, India
*Corresponding author
Abstract:

Bloodstream infections (BSIs) are the most common type of HAI occurring in the ICU. Infections associated with CVCs are categorized in the literature as either “Central line related bloodstream infections (CLRBSI), or central line associated blood stream infection (CLABSI) based on whether surveillance or ascertainment of infection is the desired goal. Blood stream infection related to central venous Catheter constitutes one of the major nosocomial device associated infection. To isolate the organisms that causing CLABSI & CLRBSI. To Determine Antimicrobial Susceptibility of the isolates & its relevance to biofilm formation. Catheter tips were processed using Extraluminal Maki’s roll over method and Endoluminal catheter flush culture methods. And simultaneously obtaining blood culture from peripheral site & from the CVC. Biofilm formation was detected by using tissue culture plate (TCP). Blood Samples and Catheter tips were collected during the period 3 year from September 2021 to September 2024 at SSB Hospital & NMC Hospital, Government Medical College, Kota, Rajasthan. A total of 155 patients with a cumulative 1380 CVC days were included. Antibiotics resistance was seen more in Biofilm producing bacteria as compared with Non biofilm producing bacteria. Catheter colonization and duration of catheterization has an important role in development of CLRBSI and CLABSI which may lead to septicaemia and multiorgan failure. The findings from this study will help to implement educational programs on CLRBSI/CLABSI for health personnel and help to initiate infection control interventions at the earliest.


Keywords: Catheter related bloodstream infection, Catheter associated blood stream infection, Antibiotic sensitivity Testing, Biofilm formation methods


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How to cite this article:

Dharmendra Singh and Anita E. Chand. 2025. Effect of Biofilm Formation on Antibiotic Resistance in Gram Positive Organisms Isolated from Central Line Associated and Central Line Related Blood Stream Infection At A Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 14(3): 98-104. doi: https://doi.org/10.20546/ijcmas.2025.1403.013
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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