Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2023 - IJCMAS--ICV 2023: 95.56 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Login as a Reviewer


See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 14, Issue:3, March, 2025

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2025.14(3): 6-9
DOI: https://doi.org/10.20546/ijcmas.2025.1403.002


Rapid Identification of Brucella melitensis using BacT/Alert Blood Culture System: A Case Report
A. Benedict Vinothini1, Sreerama Damini2, Hyma Kooleri Padinjare Veetil1*, Vonteddu Sai Pranav Reddy2, G. Varthana1, Archana Murali1Mukta Wyawahare2 and Apurba Sankar Sastry1
Department of Microbiology, Department of Medicine, JIPMER, Puducherry-605006, India
*Corresponding author
Abstract:

Brucella melitensis, a gram-negative coccobacillus, is the causative agent of the zoonotic disease brucellosis. Brucellosis can present with a variety of signs and symptoms hence the diagnosis becomes difficult. To ensure that the patient receives the appropriate treatment, laboratory testing is necessary to confirm the diagnosis. The advent of automated systems has made the identification of brucellosis easier. This facilitates the early initiation of appropriate antibiotics, thereby improving clinical outcome.


Keywords: Brucella melitensis, blood culture, BacT/Alert


References:

Baysallar M, Aydogan H, Kilic A, Kucukkaraaslan A, Senses Z, Doganci L. Evaluation of the BacT/ALERT and BACTEC 9240 automated blood culture systems for growth time of Brucella species in a Turkish tertiary hospital. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research. 2006 Jun 28;12(7):BR235-8.

Christoforidou S, Kyritsi M, Boukouvala E, Ekateriniadou L, Zdragas A, Samouris G, et al., Identification of Brucella spp. isolates and discrimination from the vaccine strain Rev.1 by MALDI-TOF mass spectrometry. Mol Cell Probes. 2020;51:101533.

https://doi.org/10.1016/j.mcp.2020.101533

Guzmán-Bracho C, Salgado-Jiménez B, Beltrán-Parra LG, Hernández-Monroy I, Vargas-Pino F, Rodríguez D, et al., Evaluation of serological diagnostic tests of human brucellosis for prevention and control in Mexico. European Journal of Clinical Microbiology & Infectious Diseases. 2020;39:575–81. https://doi.org/10.1007/s10096-019-03760-3

Kumari R, Kalyan RK, Jahan A, Jain A, Kumar P, Gupta KK, et al., Human Brucellosis: An Observational Study From a Tertiary Care Centre in North India. Cureus. 2023; https://doi.org/10.7759/cureus.42980

Larry Jameson J. Harrison’s Principles of Internal Medicine. 20th ed. J. Larry Jameson, Dennis L. Kasper, Dan L. Longo, Anthony S. Fauci, Stephen L. Hauser, Joseph Loscalzo, editors. Vol. 1. McGraw Hill Education; 2018.

Mantur BG, Amarnath SK. Brucellosis in India — a review. J Biosci. 2008;33:539–47. https://doi.org/10.1007/s12038-008-0072-1

Özkurt Z, Erol S, Tasyaran MA, Kaya A. Detection of Brucella melitensis by the BacT/Alert automated system and Brucella broth culture. Clinical microbiology and infection. 2002 Nov 1;8(11):749-52. https://doi.org/10.1046/j.1469-0691.2002.00471.x

Pathak AD, Dubal ZB, Doijad S, Raorane A, Rodrigues S, Naik R, et al., Human brucellosis among pyrexia of unknown origin cases and occupationally exposed individuals in Goa Region, India. Emerg Health Threats J. 2014;7:23846. eCollection 2014 https://doi.org/10.3402/ehtj.v7.23846

Procop GW, CDL, HGS, KEW. Koneman’s Color Atlas & Textbook of Diagnostic Microbiology. 7 th. Wolters Kluwer; 2017.

Roiz MP, Peralta FG, Valle R, Arjona R. Microbiological Diagnosis of Brucellosis. J Clin Microbiol. 1998;36:1819–1819. https://doi.org/10.1128/JCM.36.6.1819-1819.1998

Rudrik JT, Soehnlen MK, Perry MJ, Sullivan MM, Reiter-Kintz W, Lee PA, et al., Safety and Accuracy of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identification of Highly Pathogenic Organisms. J Clin Microbiol. 2017;55:3513–29. https://doi.org/10.1128/JCM.01023-17

Stephen S, Pradeep J, Sangeetha B, Jayaraman S. Seroprevalence of Human Brucellosis in and Around Puducherry: Application of an Immunocapture Agglutination Technique (Brucellacapt). J Pure Appl Microbiol. 2019;13:313–7. https://dx.doi.org/10.22207/JPAM.13.1.34

Yagupsky P, Morata P, Colmenero JD. Laboratory Diagnosis of Human Brucellosis. Clin Microbiol Rev. 2019;33. https://doi.org/10.1128/CMR.00073-19


Download this article as Download

How to cite this article:

Benedict Vinothini, A., Sreerama Damini, Hyma Kooleri Padinjare Veetil, Vonteddu Sai Pranav Reddy, G. Varthana, Archana Murali1, Mukta Wyawahare and Apurba Sankar Sastry. 2025. Rapid Identification of Brucella melitensis using BacT/Alert Blood Culture System: A Case Report.Int.J.Curr.Microbiol.App.Sci. 14(3): 6-9. doi: https://doi.org/10.20546/ijcmas.2025.1403.002
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

Citations