National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This research highlights the untapped potential of marine sediment-derived Streptomyces sp. as a prolific source of novel secondary metabolites exhibiting remarkable antibacterial and biofilm-inhibiting activities. Recognizing the rich biodiversity of marine sediments, samples were serially diluted and cultured using SCA media. After 14 days of incubation at 28ºC, distinct colonies characteristic of Streptomyces sp. was isolated for further study. Secondary metabolites were extracted using methanol and subsequently analyzed through 16S rDNA sequencing, FTIR, and GC-MS techniques. The sequencing provided insights into phylogenetic relationships, while FTIR identified key chemical bonding structures in the extracts. GC-MS analysis unveiled various volatile compounds, corroborating the bioactive potential. Antibacterial assays revealed significant zones of inhibition, confirming the efficacy of the extracts, and antibiofilm assays demonstrated notable inhibition percentages. These findings underscore the promising industrial applications of Streptomyces sp. from marine sediments, paving the way for future exploration and development of these novel secondary metabolites.
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