National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Rice (Oryza sativa L.) is one of the most important field crop after wheat in the world providing staple food to the millions. Drought is the most important environmental stress on rice and many efforts have been made to improve crop yield under drought. The present study reveals the response of rice for the drought stress at germination to seedling stages. Mannitol was selected for inducing osmotic stress. For in vitro screening of PPFM isolates for water stress tolerance, 20 isolates of PPFM from paddy were selected from the previous study of M.Sc. (Ag.) thesis work conducted in the Department of Agricultural Microbiology, Vellayani during 2015-2017 on the basis of carotenoid pigment production, IAA production, proline content, seedling vigour index and yield. These isolates were screened by paper towel method for water stress tolerance under in vitro conditions using mannitol for inducing osmotic stress. There were 21 isolates (20 KAU isolates of PPFM and one TNAU isolate) and four water stress levels (1%, 2%, 3% mannitol and control).Osmotic stress was higher in 3 per cent mannitol treatment. Seeds treated with PPFM 26 recorded the highest germination percentage, shoot length and seedling vigour index. The highest root length and shoot dry weight were observed with the isolate PPFM 15 whereas the highest root dry weight was recorded with PPFM 9. Scoring was done to assess the best five isolates and those with higher ranks were selected as better water stress tolerance capacity. Consequently, PPFM 26, PPFM 15, PPFM 38, PPFM 37 and PPFM 35 which secured ranks from 1to 5 were selected as better water stress tolerance.
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