National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of the study was to detect the tet A gene coding for tetracycline resistance in ESBL-producing strains of Escherichia coli isolated from faecal waste from pigs used as manure. Thirty-five E. coli strains containing genes coding for ESBL resistance were used in the study. These strains were isolated from faecal waste from pigs used to produce manure. They were identified using biochemical tests. An antibiotic susceptibility test was carried out using the agar diffusion antibiogram technique to determine antibiotic resistance profiles and confirmed the presence of ESBL production. PCR was carried out to search for the tet A gene coding for tetracycline resistance in strains likely to produce ESBL. Antibiogram results of the 35 E. coli strains confirmed the ESBL phenotype. These strains were resistant to several antibiotics. The rate of resistance to tetracycline was 100%. All these strains harboured the tet A resistance gene. They also presented five genetic combinations, the most predominant of which was bla TEM, tet A with a frequency of 38%. The detection of tet A resistance genes in E. coli represents a risk to public health. Resistance genes circulating in the digestive flora of farm animals therefore need to be monitored to prevent their spread.
Aarestrup, F.M. 2006. Antimicrobial Resistance in Bacteria of Animal Origin, ASM Press, Washington DC, 2006.
Agerso Y. and Sandvang D. 2005. Class 1 integrons and tetracycline resistance genes in Alcaligenes, Arthrobacter, and Pseudomonas spp. isolated from pigsties and manured soil. Applied Environnental Microbiology. 71: 7941-7947. https://doi.org/10.1128/AEM.71.12.7941-7947.2005
Agersø, Y. and Aarestrup, F. M. 2013. Voluntary ban on cephalosporin use in Danish pig production has effectively reduced extended-spectrum cephalosporinase-producing Escherichia coli in slaughter pigs. Journal of Antimicrobial and Chemotherapy. 68(3):569–572. https://doi.org/10.1093/jac/dks427
Anses-ANMV. 2014. Suivi des ventes de médicaments vétérinaires contenant des antibiotiques en France en 2013. Volumes et estimation de l’exposition des animaux aux antibiotiques. Edit.,A., ed. Maisons-Alfort. (www.anses.fr).
Bagalwa M., Karume K., Mushagalusa N G., Ndegeyi K., Birali M., Zirirane N., Macheka Z et Bayongwa C. 2013. Risques potentiels des déchets domestiques sur la santé des populations en milieu rural: cas d'Irhambi Katana (Sud-Kivu, République Démocratique du Congo). Vertigo 13:2 https://doi.org/10.4000/vertigo.14085
Balasubramaniam A, Arthanari Eswaran M, Suresh P, Sukumar K 2014. Detection of tetracycline resistance determinant tet A gene and antimicrobial resistance pattern in Escherichia coli isolates recovered from healthy layer chickens, Veterinary World. 7(9):635-638. https://doi.org/10.14202/vetworld.2014.635-638
Belloc C., Lam D. C., Pellerin J. L., Beaudeau F., Laval A. 2005. Effect of quinolone treatment on selection and persistence of quinolone-resistant Escherichia coli in swine faecal flora. Journal of Applied Microbiology. 99:954–959. https://doi.org/10.1111/j.1365-2672.2005.02667.x.
Bibbal D., Dupouy V, Prere M. F., Toutain P. L., Bousquet-Melou A. 2009. Relatedness of Escherichia coli strains with different susceptibility phenotypes isolated from swine feces during ampicillin treatment. Applied and Environmental Microbiology.75 (10):2999-3006 https://doi.org/10.1128/AEM.02143-08
Blanchard M. 2010. Gestion de la fertilité des sols et rôle du troupeau dans les systèmes coton-céréales-élevage au Mali-Sud. Savoirs techniques locaux et pratiques d’intégration agriculture élevage. Thèse Doctorat. Université Paris-Est, Créteil Val de Marne.12p
Blanco, J. E., Blanco, M., Mora, A. and Blanco, J. 1997. Prevalence of bacterial resistance to quinolones and other antimicrobials among avian Escherichia coli strains isolated from septicemic and healthy chickens in Spain. Journal of Clinical Microbiology. 35(8): 2184-2185. https://doi.org/10.1128/jcm.35.8.2184-2185.1997
Daini, O. A. and Adesemowo, A. 2008. Antimicrobial susceptibility pattern and R plasmid of clinical strains of Escherichia coli. Australian Journal of Basic Applied Sciences. 2: 397-400.
EFSA. 2011. Scientific opinion on the public health risks of bacterial strains producing extended-spectrum beta-lactamases and/or AmpC beta-lactamases in food and food producing animals. EFSA Journal, 9(2322). https://doi.org/10.2903/j.efsa.2011.2322
Enne V, Cassar C, Sprigings K, Martin J. Woodward, Peter M. Bennett M. 2007. A high prevalence of antimicrobial resistant Escherichia coli isolated from pigs and a low prevalence of antimicrobial resistant E. coli from cattle and sheep in Great Britain at slaughter. FEMS Microbiology Letters. 278:193–199. https://doi.org/10.1111/j.1574-6968.2007.00991.x
FAO, 2006. Livestock's long shadow. Environmental issues and options. Rapport de la FAO. Rome: FAO. 390p
Farmer. (1999). Enterobacteriaceae: Introduction and identification In: Manual of clinical Microbiology, P. R. Murray, E. J. Baron, M. A. Pfatter, Tenoven F. C. and R. H. Yolken (eds) 7th ed. American Society for Microbiology. 442-458
Gomgnimbou A. P. K., Nacro H. B., Sanon O. H., Sieza I., Kiendrebeogo T., Sedogo M. P. et Martinez J. 2014. La gestion des déjections animales dans la zone périurbaine de Bobo-Dioulasso (Burkina Faso): structure des élevages, perception de leur impact environnemental et sanitaire, perspectives. Cahiers Agricultures, vol. 23, n°6. 345-406 p
Hu G Z, Pan Y S, Wu H, Hu H, Xu R, Yuan L, Liu J L, Feng J K. 2013. Prevalence of tetracycline resistance genes and identification of tet(M) in clinical isolates of Escherichia coli from sick ducks in China. Journal of Medical Microbiology. 62, 851–858 https://doi.org/10.1099/jmm.0.051896-0
Hutchison, M. L., Walters, L. D., Avery, S. M., Synge, B. A., Moore, A. 2004. Levels of zoonotic agents in British livestock manures. Lett. Appl. Microbiol. 39 (2), 207–214. https://doi.org/10.1111/j.1472-765X.2004.01564.x
Karczmarczyk, M., Walsh, C., Slowey R., Leonard N., and Fanning, S. 2011. Molecular characterization of multidrug- resistant Escherichia coli isolates from Irish cattle farms. Applied of Environmental Microbiology. 77(20): 7121-7127. https://doi.org/10.1128/AEM.00601-11
Koo H. and Woo G. 2011. Distribution and transferability of tetracycline resistance determinants in Escherichia coli isolated from meat and meat products. International Journal of Food Microbiology. 145 (2-3): 407-413. https://doi.org/10.1016/j.ijfoodmicro.2011.01.003
Lanz R., Kuhnert P., Boerlin P. 2003. Antimicrobial resistance and resistance gene determinants in clinical Escherichia coli from different animal species in Switzerland. Vetenary Microbiology. 91: 73– 84. https://doi.org/10.1016/s0378-1135(02)00263-8
Mali. Meadr, 2015. Ministère de l’environnement, de l’assainissement et du développement durable. Rapport troisième communication nationale du Mali à la convention cadre des nations unies sur les changements climatiques.15 P
Manyi-Loh C. E., Mamphweli S. N., Meyer E. L., Doré S., Simon M. et Okoh A. I. 2016. Un aperçu du contrôle des bactéries pathogènes dans le fumier de bétail. Int. J. Environ. Rés. Santé publique, 13, no. 9: 843p.
McEwen S. 2002. Uses of antimicrobials in food animals in Canada: impact on resistance and human health. Report of the Advisory Committee on Animal Uses of Antimicrobials and Impact on Resistance and Human HealthVeterinary Drugs Directorate, Health Canada.., ed. 75p.
Muhammad A. A., Hassan, S. M. R., Alam, S. and Shirin, M. 2009. Antibiotic resistance of Escherichia coli isolated from poultry and poultry environment of Bangladesh. American Journal of Environmental Science. 5(1): 47-52. https://doi.org/10.3844/ajessp.2009.47.52
Phillips I. and Casewell M. 2004. "Does the use of antibiotics in food animals pose a risk to human health? A critical review of published data."Journal of Antimicrobial Chemotherapy. 53 (1): 28-52 https://doi.org/10.1093/jac/dkg483
Sanders P., Bousquet-Mélou A., Chauvin C., Toutain, P. L. 2011. Utilisation des antibiotiques en élevage et enjeux de santé publique. INRA Production Animale. 24 (2): 199–204. https://doi.org/10.20870/productions-animales.2011.24.2.3254
Schwaiger K., lzel C., Bauer J. 2010. Resistance gene patterns of tetracycline resistant Escherichia coli of human and porcine origin. Vetenary Microbiology. 142: 329–336. https://doi.org/10.1016/j.vetmic.2009.09.066
Sorum H. and Sunde M. 2001. Resistance to antibiotics in the normal flora of animals. Vetenary Research. 32:227-241. https://doi.org/10.1051/vetres:2001121
Stine M. S. and Morris J. G. 2007. Widespread distribution of tetracycline resistance genes in a confined animal feeding facility. International Journal of Antimicrobial Agents. 29: 348-352. https://doi.org/10.1016/j.ijantimicag.2006.11.015
Szczepanowski R., Krahn I., Linke B. 2004. Antibiotic multiresistance plasmid pRSB101 isolated from a wastewater treatment plant is related to plasmids residing in phytopathogenic bacteria and carries eight different resistance determinants including a multi-drug transport system. Microbiology. 150: 3613-3630. https://doi.org/10.1099/mic.0.27317-0
Wegener H. G., Aarestrup F. M., Gerner-Smidt P., Bager F. 1999. Transfer of antibiotic resistant bacteria from animals to man. Acta Veterinaria. Scandinavica Supplementum. 92: 51-57.
Zhang C. P., Ning Yi-B., Song Li. 2010. Resistance to tetracycline and distribution of tetracycline resistance determinants in commensal Escherichia coli isolated from clinically healthy chickens and Pigs. Sci Agric Sin. 43:2578-2583. https://doi.org/10.3864/j.issn.0578-1752.2010.12.024
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