National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The current study focuses on the screening and identification of halophilic bacteria from different habitats of Odisha for selecting a potentmulti-metal resistance bacterium. The halophilic bacteria were isolated and screened with NaCl concentration using 0%-24% (w/v). The selected halophiles were studied for their growth, colony morphology, sodium content, flavonoid content and multi-metal resistance. The isolated bacteria were found to be moderately halophilic to slight halophilic in nature due to the presence of salt such as sodium, magnesium and bicarbonate in the collected samples. The result showed the utmost number of viable cells (1.65 to 5.40 cfu/mL) is due to the hypersaline nature making the bacterial cells suitable to grow under 15%-18% (w/v) NaCl concentration. They were found to be gram-positive and organized in single rods and clusters. The presence of sodium (0.018-0.249 mg/L) were confirmed with the help of flame photometry in the halophiles. The flavonoid content (0.074-0.330 mg/mL) indicated the presence of antioxidant activity.AS’S-I strain found to be potent multi-metal resistant halophilic bacteria analysed through SEM analysis. Molecular identification of AS’S-I confirmed the Halomonassp. The study suggests that AS’S-I halophilic bacteria could be used for the multi-metal removal.
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