National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Gastric ulcers represent a significant global health burden, affecting millions of individuals annually. Helicobacter pylori is an important pathogen in humans and is considered the main cause of refractory gastritis. It is also associated with the development of peptic ulcers, atrophic gastritis, and gastric tumors. This study aimed to detect the presence of H. pylori in stool samples from patients with gastric ulcers using polymerase chain reaction (PCR) and targeting the cagA, vacA, and ureC genes. Stool samples were collected from 200 symptomatic patients and subjected to culture analysis to isolate H. pylori. The presence of H. pylori isolates was confirmed by culture analysis, Gram staining, biochemical assays, and PCR tests targeting the cagA, vacA, and ureC genes. Of the 200 stool samples, 125 showed positive results for H. pylori colonies in males and 75 in females on agar plates., which were confirmed by Gram staining and biochemical assays. Genomic DNA of Helicobacter pylori was taken based on the cetyl trimethyl ammonium bromide protocol (CTAB). Nested PCR was performed using specific primers (HP-F and HP-R), (ccagA-F and cagA-R), (vacAs1 and vacAs2), (vacAm1 and vacAm2), and (VacAF and VacAR) sets for the ureC gene, as well as the virulence genes of cagA and vacA. Gel electrophoresis analysis was performed to visualize the PCR products. PCR results showed the presence of cagA, vacA, and ureC genes in H. pylori isolates. These results indicate the prevalence of H. pylori infection and the presence of virulence genes associated with peptic ulcer disease in the study population.
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