National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study explores the phytochemical composition and antimicrobial properties of Premna tomentosa leaf extracts using different solvents: methanol, chloroform, ethyl acetate, and petroleum ether. The phytochemical analysis revealed that methanol was the most effective solvent, extracting the highest concentrations of alkaloids (450 mg/g equivalent), flavonoids (650 mg/g equivalent), phenols (550 mg/g equivalent), and tannins (500 mg/g equivalent). Chloroform also showed considerable efficacy, particularly in extracting flavonoids (650 mg/g equivalent) and alkaloids (400 mg/g equivalent). The antimicrobial activity of the extracts was tested against four bacterial strains (Pseudomonas fluorescens, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis) and three fungal strains (Fusarium oxysporum, Sclerotium rolfsii, and Phytophthora infestans). The methanol extract exhibited the highest antibacterial activity, with zones of inhibition measuring 12 mm against Pseudomonas fluorescens, 9 mm against E. coli, 6 mm against Staphylococcus aureus, and 5 mm against Bacillus subtilis. The chloroform extract also showed significant antibacterial effects, with inhibition zones ranging from 5 mm to 7 mm across different bacterial strains. For antifungal activity, the methanol extract again demonstrated the highest efficacy, with 60% inhibition against both Fusarium oxysporum and Phytophthora infestans, and 50% inhibition against Sclerotium rolfsii. Chloroform and ethyl acetate extracts showed moderate antifungal activity, while petroleum ether extract was the least effective. The study underscores the potential of Premna tomentosa for developing natural antimicrobial agents and highlights the importance of solvent selection in maximizing the extraction of these compounds.
Chang, C., Yang, M., Wen, H., & Chern, J. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3), 178-182.
Chikezie, P. C., Ibegbulem, C. O., & Mbagwu, F. N. (2020). Bioactive principles from medicinal plants. Research Journal of Phytochemistry, 9, 88-115. https://doi.org/10.3923/rjphyto.2015.88.115
Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4), 564-582. https://doi.org/10.1128/cmr.12.4.564
Eloff, J. N. (1998). Which extractant should be used for the screening and isolation of antimicrobial components from plants? Journal of Ethnopharmacology, 60(1), 1-8. https://doi.org/10.1016/s0378-8741(97)00123-2
Gaurav, K., Saklani, S., & Rani, R. (2011). Phytochemical screening and GC-MS analysis of the methanolic fruit extract of Momordica charantia. Journal of Chemical and Pharmaceutical Research, 3(2), 104-112.
Ghalem, B. R., & Mohamed, B. (2008). Antibacterial activity of leaf extract of Eucalyptus globulus (Myrtaceae) against Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, and Bacillus cereus. Asian Pacific Journal of Tropical Biomedicine, 1(3), 73-76.
Ghasemzadeh, A., & Ghasemzadeh, N. (2011). Flavonoids and phenolic acids: Role and biochemical activity in plants and human. Journal of Medicinal Plants Research, 5(31), 6697-6703. https://doi.org/10.5897/JMPR11.1404
Hajdú, Z., Hohmann, J., Forgo, P., Martinek, T., Dervarics, M., Zupkó, I. (2021). Phytochemical screening and antioxidant and antimicrobial activities of selected mangrove species. Archives of Microbiology, 21(4), 391–394.
Hameed, A., Hussain, S. A., Yang, J., Ijaz, M. U., Liu, Q., Suleria, H. A. R., & Song, Y. (2021). Antioxidants potential of filamentous fungi: a review. Nutrients, 13(2), 110.
Harborne, J. B. (1998). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis (3rd ed.). Springer Science & Business Media.
Makkar, H. P. S., Blümmel, M., & Becker, K. (1993). Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. Journal of the Science of Food and Agriculture, 61(2), 161-165. https://doi.org/10.1002/jsfa.2740610205
Nithya, V., Sangeetha, S., & Dhanalakshmi, D. (2021). Antimicrobial and antioxidant activities of bioactive compounds derived from Premna tomentosa leaves. Journal of Herbal Medicine, 28, 100437.
Nostro, A., Germano, M. P., D’Angelo, V., Marino, A., & Cannatelli, M. A. (2000). Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Letters in Applied Microbiology, 30(5), 379-384. https://doi.org/10.1046/j.1472-765x.2000.00731.x
Pandey, A. K., & Tripathi, N. N. (2011). Antifungal properties of some selected plant extracts against Fusarium oxysporum f. sp. ciceri. Asian Journal of Plant Science and Research, 1(4), 22-27.
Parekh, J., Jadeja, D., & Chanda, S. (2005). Efficacy of aqueous and methanol extracts of some medicinal plants for potential antibacterial activity. Turkish Journal of Biology, 29(4), 203-210.
Rabe, T., & Van Staden, J. (1997). Antibacterial activity of South African plants used for medicinal purposes. Journal of Ethnopharmacology, 56(1), 81-87. https://doi.org/10.1016/s0378-8741(96)01515-2.
Redfern, J., Kinninmonth, M., Burdass, D., & Verran, J. (2014). Using Soxhlet ethanol extraction to produce and test plant material (Essential Oils) for their antimicrobial properties. Journal of Microbiology & Biology Education, 15(1), 45-46. https://doi.org/10.1128/jmbe.v15i1.656
Riaz, T., Abbasi, M. A., Aziz-ur-Rehman, Shahzadi, S., & Malik, S. A. (2021). Phytochemical, antioxidant and antimicrobial activity studies of different extracts and their fractions from a native plant of Pakistan: Quercus baloot. Natural Product Research, 35(20), 3771-3775.
Salazar-Aranda, R., Pérez-López, L. A., López-Arroyo, J., & Waksman de Torres, N. (2020). Antifungal activity of selected plant polyphenols against Fusarium oxysporum and Aspergillus niger. Phytotherapy Research, 34(5), 1127-1132.
Saxena, M., Saxena, J., Nema, R., Singh, D., & Gupta, A. (2023). Phytochemistry of medicinal plants. Journal of Pharmacognosy and Phytochemistry, 15(1), 168-182.
Shamsa, F., Monsef, H., Ghamooshi, R., & Verdian-rizi, M. (2008). Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai Journal of Pharmaceutical Sciences, 32(1), 17-20.
Sharma, P., Sharma, R., & Jain, R. (2022). Antifungal and antioxidant activity of aqueous extract of selected plants against phytopathogens. Journal of Plant Pathology, 104(2), 347-356.
Singh, P., Kumar, V., & Sharma, R. (2012). In vitro evaluation of antifungal activity of neem and garlic extracts against Fusarium oxysporum f. sp. ciceri. Journal of Agricultural Technology, 8(1), 209-218.
Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178 https://doi.org/10.1016/S0076-6879(99)99017-1
Sivakumar, D., & Bautista-Baños, S. (2020). A review on the use of essential oils for postharvest decay control and maintenance of fruit quality during storage. Crop Protection, 134, 104831.
Ventola, C. L. (2020). The antibiotic resistance crisis: part 1: causes and threats. Pharmacy and Therapeutics, 45(4), 277-283.
Wang, X., Shen, Y., Zhang, C., Zeng, J., Li, Y., Wang, S, & Wang, Y. (2021). Terpenoids: natural products for controlling plant pathogens. Plant Disease, 105(6), 1707-1716.
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