National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study aims to investigate two of the Uropathogenic Escherichia coli (UPEC) pathogenesis mechanisms, as adherence and invasion in primate kidney epithelial (Vero) cells. Methods: 20 E. coli isolates from outpatients with UTI in one public clinic were evaluated for adherence and invasive abilities in Vero cells. Results: All 18 positive isolates for fimH gene adhered to the Vero cells (100%). The two UPEC isolates that did not carry the fimH gene did not adhere. Two different UPEC adherence patterns were observed: aggregative adherence pattern (AA) and a pattern called “no typical adherence pattern” (NTA). Among 18 adherent UPEC isolates, 11 presented AA adherence pattern (61%) and 7 showed NTA pattern (39%). The high rate of adherence of the UPEC fimH positive isolates (≅90%) to Vero cells suggests the expression and participation of the FimH adhesin in UPEC adherence behavior. Invasive capacity of UPEC isolates ranged of about 6.5% to 15.33%. Results corroborate the fact that UPEC can persist in the urinary tract through its adhesion, as well as invade cells establishing the bacterial UTI process.
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