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Original Research Articles                      Volume : 13, Issue:4, April, 2024

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2024.13(4): 1-7
DOI: https://doi.org/10.20546/ijcmas.2024.1304.001


Transdermal Delivery of Paclitaxel-Anionic Nanoparticles to Epidermis Layer, Pterostilbene, and Pterostilbene glycoside, and Their Application for Treatment of Skin Cancer and Wrinkle
Hiroki Hamada1*, Daisuke Uesugi1, Kohji Ishihara2, Ryusuke Hosoda2, Kei Shimoda3,Atsuhito Kuboki2, Noriyuki Uchida4 and Yuya Kiriake5
1Meisterbio Co. Ltd., 29-13Ekimoto-cho, Kita-ku, Okayama 700-0024, Japan
2Department of Biological Science, Faculty of Life Science, Okayama University of Science,1-1 Ridai-cho, Kita-ku, Okayama 700-0005, Japan
3Department of Biomedical Chemistry, Faculty of Medicine, Oita University, 1-1 Hasama-machi,Oita 879-5593, Japan
4RIKEN Center for Emergent Matter Science, Wako, Saitama, Japan
5Faculty of Medicine and Health Sciences, Yamaguchi University, 1-1-1 Minamikogushi,Ube-shi, Yamaguchi 755-8505, Japan
*Corresponding author
Abstract:

Composite nanoparticles, “anionic bicelles”, composed of anionic phospholipid of 1,2- dipalmitoyl- sn-glycero-3-phosphorylglycerol (DPPG) and paclitaxel were prepared by mixing them in water and a subsequent heating/cooling/ultrasonicating process. Anionic DPPG-paclitaxel nanoparticles (particle size: 12 nm) could be prepared after ultrasonic fragmentation at low temperature of 4°C. Upon addition of fluorescently labeled paclitaxel nanoparticles stabilized with DPPG to rat skin tissue (in vitro), the nanoparticles “anionic bicelles” (particle size: 12 nm) infiltrated into the epidermis layer penetrating stratum corneum (intercellular space: ca. 100 nm). In addition, during the anti-skin cancer test using mouse model of skin cancer, our study revealed that the numbers of papillomas of the mouse applied with paclitaxel nanoparticles stabilized with DPPG, “anionic bicelles”, to mouse skin were decreased, although those of the mouse applied with paclitaxel itself to mouse skin (control) were increased. Thus, this study established that since paclitaxel nanoparticles stabilized with DPPG could permeate stratum corneum and be incorporated into the epidermis layer of mouse, they could also treat skin cancer (in vivo). On the other hand, a mixture of anionic phospholipids, sodium cholate and Technol PG, allowed for formation of anionic nanoparticles, “anionic liposomes”, (particle size: 3 nm). This study established that as pterostilbene and pterostilbene glycoside could permeate stratum corneum and be incorporated into the epidermis layer, they could also treat wrinkle.


Keywords: DPPG-paclitaxel nanoparticles, Anionic phospholipid, Epidermis layer, Permeation of Stratum corneum, Treatment of skin cancer, Anti-skin cancer effect, Anti-wrinkle effect


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How to cite this article:

Hiroki Hamada, Daisuke Uesugi, Kohji Ishihara, Ryusuke Hosoda, Kei Shimoda, Atsuhito Kuboki, Noriyuki Uchida and Yuya Kiriake. 2024. Transdermal Delivery of Paclitaxel-Anionic Nanoparticles to Epidermis Layer, Pterostilbene, and Pterostilbene glycoside, and Their Application for Treatment of Skin Cancer and Wrinkle.Int.J.Curr.Microbiol.App.Sci. 13(4): 1-7. doi: https://doi.org/10.20546/ijcmas.2024.1304.001
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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