National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Antibiotic is one of the most significant discoveries and have brought a revolution in the field of medicine for human therapy. In developing nations, antibiotics use have helped to increase the life expectancy by lowering the deaths due to bacterial infections, but the risks associated with antibiotics pollution is largely affecting people. Since antibiotics are released partially degraded and undegraded into environment creating antibiotic pollution, and its bioremediation is a challenging task. The present study aims to isolate and acclimatize antibiotic-degrading bacterial strains for Norfloxacin, Levofloxacin, Amoxicillin and Cloxacillin from the contaminated soil of pharmaceutical waste site. Bacterial strains were isolated and acclimatized by continuous enrichment of cultures with antibiotics as the sole carbon source. The antibiotic susceptibility test, thiol mercury salt ultraviolet spectrophotometry (TMSUS), morphological observations, and 16SrDNA sequence analysis were used to identify and characterize the isolated strains. Three bacterial isolates (A, B and F) were obtained, and two of them (A, B) with the highest degradation rates were identified to belong to the same genera as Bacillus. These two isolates were found to be resistant to antibiotic in an antibiotic sensitivity test. The TMSUS indicated that the strains A and B had good performance in amoxicillin degradation. Two bacterial strains isolated from the pharmaceutical waste site are effective in degrading antibiotic and can be potentially used for bioremediation of antibiotic-contaminated soils.
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