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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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Original Research Articles                      Volume : 12, Issue:3, March, 2023

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2023.12(3): 256-263
DOI: https://doi.org/10.20546/ijcmas.2023.1203.031


RAPD Marker Based Determination of Genetic Variability among Trichoderma Isolates
R. Thilagavathi*
Department of Plant Pathology, Agricultural College and Research Institute, TNAU, Kudumiyanmalai, India
*Corresponding author
Abstract:

Twenty Trichoderma isolates collected from different geographical locations of Tamil Nadu were studied for their genetic relationship. The results showed that the PCR-based Random Amplified Polymorphic DNA (RAPD) marker produced DNA fragments of 0.125 kb to 10 kb size with polymorphism while employing five random primers. The dendrogram constructed using Unweighted Pair Group Method of Arithmetic means (UPGMA) produced two main clusters of Trichoderma isolates. Cluster 1 bears 5 isolates with 60 per cent similarity coefficient between them and cluster 2 bears 15 isolates with 57 per cent similarity. Overall, all the isolates have about 49 per cent similarity coefficient indicating that they were genetically varied by their unique banding patterns. Such variability has opened new possibility of using most efficient and more isolates of Trichoderma in the preparation of eco-friendly bio-pesticide.


Keywords: Genomic variation - RAPD marker – Trichoderma isolates, soil and root ecosystems

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How to cite this article:

Thilagavathi, R. 2023. RAPD Marker Based Determination of Genetic Variability among Trichoderma Isolates.Int.J.Curr.Microbiol.App.Sci. 12(3): 256-263. doi: https://doi.org/10.20546/ijcmas.2023.1203.031
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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