National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Hospital Acquired Infections are the rising threat in the health care facilities across the globe. Multidrug resistance is a serious problem all across the world. The ESKAPE (Enterococcus faecium, Staphylococcus aureus including MRSA, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species) pathogens in the hospital are the commonly acquired infections. As most ICU patients are frequently on broad spectrum antibiotics this induces selective antibiotic pressure which leads to development of antimicrobial resistance among the microorganisms of various ICU. Aim: To determine the prevalence of ESKAPE pathogens isolated from blood culture of patients admitted in various Intensive Care Units. This was a cross-sectional study conducted in the Department of Microbiology and Intensive care units (MICU, PICU and NICU) at UCMS & GTBH; Delhi was conducted from January 2021 to April 2022. The Inadequate quantity of sample and contaminated blood culture samples were not enrolled for this study. The antibiotic susceptibility pattern of the isolated organisms was performed by Kirby–Bauer disk diffusion method on mueller–Hinton agar plates, and the results were recorded as per the CLSI 2022 guidelines. Out of these 611 blood culture samples growth of microorganisms was observed in 115 (18.82%) samples. 496 (81.18%) blood culture samples showed no growth. One blood culture sample was taken from each patient. These 115 blood culture isolates were further tested for species identification by standard conventional phenotypic methods and their antimicrobial susceptibility testing (AST) was performed manually by Kirby Bauer disc diffusion method. Among these, 611 blood culture samples, 350 (57.28%) samples were from NICU, 211(34.53%) were from MICU, and 72 (8.18%) were from PICU isolated respectively. Conclusion: Our results from a relatively large dataset highlight the pathogenicity of ESKAPE bacteria and quantify their impact on patient length of stay, cost of care, and mortality. We also found that amongst all blood culture orders, employing simple word algorithms can predict ESKAPE infections better than random guessing.
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