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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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Original Research Articles                      Volume : 12, Issue:3, March, 2023

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2023.12(3): 203-213
DOI: https://doi.org/10.20546/ijcmas.2023.1203.024


A study on the Prevalence of ESKAPE Pathogens Isolated from the Blood Culture Specimens of Various Intensive Care Units Patients Admitted in a Tertiary Care Hospital
Vidushi Singh1, N. P. Singh1, Kirti Nirmal1*, Shukla Das1, Bineeta Kashyap1, Prateek Singh2, Prerna Batra3 and Asha Tyagi4
1Department of Microbiology, University College of Medical Sciences and Guru Tag Bahadur, Hospital, Dilshad Garden, Delhi – 110095, India
2Department of Anesthesia, Government Medical College, Saharanpur, Uttar Pradesh.
3Department of Pediatrics,
4Department of Anesthesia, University College of Medical Sciences and Guru Tag Bahadur, Hospital, Dilshad Garden, Delhi – 110095, India
*Corresponding author
Abstract:

Hospital Acquired Infections are the rising threat in the health care facilities across the globe. Multidrug resistance is a serious problem all across the world. The ESKAPE (Enterococcus faecium, Staphylococcus aureus including MRSA, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species) pathogens in the hospital are the commonly acquired infections. As most ICU patients are frequently on broad spectrum antibiotics this induces selective antibiotic pressure which leads to development of antimicrobial resistance among the microorganisms of various ICU. Aim: To determine the prevalence of ESKAPE pathogens isolated from blood culture of patients admitted in various Intensive Care Units. This was a cross-sectional study conducted in the Department of Microbiology and Intensive care units (MICU, PICU and NICU) at UCMS & GTBH; Delhi was conducted from January 2021 to April 2022. The Inadequate quantity of sample and contaminated blood culture samples were not enrolled for this study. The antibiotic susceptibility pattern of the isolated organisms was performed by Kirby–Bauer disk diffusion method on mueller–Hinton agar plates, and the results were recorded as per the CLSI 2022 guidelines. Out of these 611 blood culture samples growth of microorganisms was observed in 115 (18.82%) samples. 496 (81.18%) blood culture samples showed no growth. One blood culture sample was taken from each patient. These 115 blood culture isolates were further tested for species identification by standard conventional phenotypic methods and their antimicrobial susceptibility testing (AST) was performed manually by Kirby Bauer disc diffusion method. Among these, 611 blood culture samples, 350 (57.28%) samples were from NICU, 211(34.53%) were from MICU, and 72 (8.18%) were from PICU isolated respectively. Conclusion: Our results from a relatively large dataset highlight the pathogenicity of ESKAPE bacteria and quantify their impact on patient length of stay, cost of care, and mortality. We also found that amongst all blood culture orders, employing simple word algorithms can predict ESKAPE infections better than random guessing.


Keywords: Blood specimens, ESKAPE pathogens, Various Intensive care units

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How to cite this article:

Vidushi Singh, N. P. Singh, Kirti Nirmal, Shukla Das, Bineeta Kashyap, Prateek Singh, Prerna Batra and Asha Tyagi. 2023. A study on the Prevalence of ESKAPE Pathogens Isolated from the Blood Culture Specimens of Various Intensive Care Units Patients Admitted in a Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 12(3): 203-213. doi: https://doi.org/10.20546/ijcmas.2023.1203.024
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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