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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 11, Issue:11, November, 2022

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(11): 117-125
DOI: https://doi.org/10.20546/ijcmas.2022.1111.014


Phenotypic Identification of Citrobacter Isolates from Cloacal Swabs of Apparently Healthy Turtles at the River Banks in Lokoja, Kogi State, Nigeria
Madubuike Stella Anelechi1*, Mailafia Samuel1, Egwu O. Godwin2, Olabode H. Olatunde1, Momoh A.Habiba3, Ode O. Julius4 and Ramon-Yusuf S.Babatunde4
1Department of Veterinary Microbiology, 2Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Abuja, FCT, Nigeria
3Federal College of Animal Health and Production Technology, Vom, Plateau State. Nigeria
4Department of Veterinary Pharmacology & Toxicology, University of Abuja, Nigeria
*Corresponding author
Abstract:

Citrobacter species are opportunistic pathogens in humans that can lead to invasive disease, including infections of the urinary tract, respiratory tract, Central Nervous System, skin, and soft tissues. A total of 245 cloacal swab samples were collected from the cloaca of apparently healthy turtles and subjected to conventional biochemical tests. Presumptive identification on MacConkey Agar (MAC) and Xylose lysine deoxycholate agar (XLD) yielded 42.0% (103) on each medium, on Eosin Methylene Blue agar (EMB) yielded 21.6 % (53), and on Salmonella-Shigella agar yielded 9.8% (24) isolates while Indole test, Methyl Red, VogesProskauer, Citrate utilization test (IMVIC) and Aesculin hydrolysis tests conformed with the conventional biochemical reactions for Citrobacter. The MicrobactTM 24 E identification test performed on the 23 isolates yielded a prevalence rate of 47.8 % for Citrobacter. However, this study is therefore the first phenotypic study to confirm the presence of Citrobacter species in turtles from the River banks in Lokoja, the study location. Humans can contract the infection through exposure, handling and consumption of poorly cooked turtle meat. Control measures are therefore necessary especially during the handling of apparently healthy turtle, to ensure that proper hygienic measures are observed.


Keywords: Citrobacter, Turtles, Lokoja, Isolates, Opportunistic, Biochemical

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How to cite this article:

Madubuike Stella Anelechi, Mailafia Samuel, Egwu O. Godwin, Olabode H. Olatunde, Momoh A.Habiba, Ode O. Julius and Ramon-Yusuf S.Babatunde. 2022. Phenotypic Identification of Citrobacter Isolates from Cloacal Swabs of Apparently Healthy Turtles at the River Banks in Lokoja, Kogi State, Nigeria.Int.J.Curr.Microbiol.App.Sci. 11(11): 117-125. doi: https://doi.org/10.20546/ijcmas.2022.1111.014
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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