National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The agriculture sector has a vital challenge in reducing its reliance on chemical pesticides. Use of plant beneficial microorganisms is considered as one of the most attractive alternatives that makes a positive contribution to environmentally safe, sustainable agriculture. Trichoderma species have proved to be one of the promising bio-control agents for the management of plant diseases, and the search for novel species of the fungal antagonist is in progression. The aim of this research work is to isolate and evaluate Trichoderma species from rhizospheric soil on five different nutritional (culture) media PDA, SDA, MSDA, CDA, and TSM. The most effective culture media for the isolation of Trichoderma isolates was PDA, followed by CDA, SDA, MSDA and TSM. The highest linear growth (84mm) was found in Potato dextrose agar and the lowest linear growth (45.66 mm) was found in TSM. The maximum biomass (fresh and dry) was found in PDB (1541.7 mg/100 ml and 749 mg/100ml, respectively).The minimum biomass (fresh and dry) was observed on TSM (677.67 mg/100 ml and 365 mg/100ml, respectively). For isolation and growth studies of antagonistic fungi, as well as for various laboratory screenings, the selection of media is an important and critical factor. The present work focuses on the selection of the best supportive medium for the isolation, growth and biomass production of selected Trichoderma harzianum for future commercialization as bio-control agent and production of industrially important enzymes (cellulase, pectinase, and amylase etc.).
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