'' '' '' '' '' Induced Expression of Ovarian Heat Shock Proteins with Response to Thermal Stress in an Endemic Freshwater Crab, <em>Sartoriana spinigera</em> (Wood-mason, 1871) from East Coast of India
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles                      Volume : 11, Issue:9, September, 2022

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(9): 244-251
DOI: https://doi.org/10.20546/ijcmas.2022.1109.027


Induced Expression of Ovarian Heat Shock Proteins with Response to Thermal Stress in an Endemic Freshwater Crab, Sartoriana spinigera (Wood-mason, 1871) from East Coast of India
Pradipta Pati1, Debasmita Dubey2 and Gopal Krishna Purohit3*
1Christ College, Kanika Road, Cuttack, Odisha – 753008, India 2Siksha O Anusandhan University, Bhubaneswar, Odisha – 751003, India 3Heredity Biosciences LLP. Plot No-818, Mayfair lagoon road, Jayadev Vihar, Bhubaneswar, Odisha – 751015, India
*Corresponding author
Abstract:

The Heat shock proteins (HSP) play an important role in controlling the apoptosis and inflammatory response. These proteins are released into the extracellular environment and act as mediators of inflammation. Protein folding accuracy is ensured by chaperone activity, which also prevents the accumulation of abnormal isoforms. In the current work, Sartoriana spinigera, an endemic freshwater crab, is examined for its survival and Hsps expression patterns in ovarian tissues in response to short-term heat stress. Crabs maintained at 25 oC served as control. Expression of Hsp genes were analyzed using semi-quantitative and quantitative PCR in both groups to see whether these crabs have lost the Hsp gene plasticity. Hsp gene expression analysis showed up-regulation of hsp90 (2.1-fold) and hsp47 (2.5-fold) in heat stressed crabs, whereas there was no alteration in expression of other Hsps. Up-regulation of all the Hsps was observed in the heat shocked crabs, whereas expression of all Hsps were down regulated to the basal level in crabs maintained at 34 oC/24 hour. These results indicate that Hsp90 and Hsp60 play important role in survival of Sartoriana spinigera under thermal stress.


Keywords: Sartoriana spinigera, Heat stress, Thermal acclimation, Hsps, RT-qPCR
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How to cite this article:

Pradipta Pati, Debasmita Dubey and Gopal Krishna Purohit. 2022. Induced Expression of Ovarian Heat Shock Proteins with Response to Thermal Stress in an Endemic Freshwater Crab, Sartoriana spinigera (Wood-mason, 1871) from East Coast of India.Int.J.Curr.Microbiol.App.Sci. 11(9): 244-251. doi: https://doi.org/10.20546/ijcmas.2022.1109.027
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.