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Original Research Articles                      Volume : 11, Issue:9, September, 2022

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(9): 197-211
DOI: https://doi.org/10.20546/ijcmas.2022.1109.023


Formulation and Stability of Clove Leaf (Syzygium aromaticum L.) Essential Oil Microemulsion
1Food Technology Study Program, 2Agroindustrial Technology Study Program, Faculty of Agricultural Technology, Udayana University, Indonesia
*Corresponding author
Abstract:

Clove leaf essential oil is a volatile compound, unstable to heat, light and air. Clove leaf essential oil can be prepared in microemulsion (o/w) preparations to maintain stability and aplicable in food. The purpose of this study was to obtain a microemulsion formulation of clove leaf essential oil (o/w) with good stability. The nonionic surfactants used consisted of Tween 20, Span 80 and Tween 80. This research was carried out in 2 stages. The first stage consisted of a mixture of surfactant ratios (Tween 20, Span 80 and Tween 80) with 5 treatment levels, namely (98:2:0), (98:0:2), (98:0,5:1.5), (98:1,5:0,5), and (98:1:1). The second stage consisted of the ratio of clove leaf essential oil and the ratio of surfactants selected in the first stage which consisted of 8 levels, namely (15:85), (17.5:82,5), (20:80), (22.5: 77.5), (25:75), (27.5:72,5), (30:70), and (32.5:67,5). Stability test was carried out on centrifugation force, heating, pH, and dilution with turbidity index parameters. The turbidity index value was below 1% and the transparent appearance was declared as a stable microemulsion. The results showed that the ratio of the mixture of nonionic surfactants (98: 0.5: 1.5) was the best result with the characteristic turbidity index value before testing 0.181±0.007%, after centrifugation 0.188±0.004% and after heating 0.215±0.013%. Meanwhile, the ratio of clove leaf essential oil to surfactant 22.5:77.5 was the best result with a transparent appearance, the turbidity index value before testing was 0.214±0.013%, after centrifugation was 0.225±0.013% and after heating was 0.268±0.012%. The clove essential oil microemulsion was stable at pH (3.5; 4.5; 5.5) and dilution (1:1, 1:9, 1:99). The size of the clove leaf essential oil microemulsion droplets formed 2 peaks, namely peak 1 of 93% with an average size of 35.4 nm and peak 2 of 7% with an average size of 2759.3 nm with a polydispersity index value of 0.420. The clove essential oil microemulsion had an average zeta potential value of 0.1 mV. Clove leaf essential oil was stable for 8 weeks of storage.


Keywords: Microemulsion, surfactant, formulation, stability, Syzygium aromaticum L

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How to cite this article:

Wayan Ryantama Swastika Braja, I., I. Dewa Gde Mayun Permana and Lutfi Suhendra. 2022. Formulation and Stability of Clove Leaf (Syzygium aromaticum L.) Essential Oil Microemulsion.Int.J.Curr.Microbiol.App.Sci. 11(9): 197-211. doi: https://doi.org/10.20546/ijcmas.2022.1109.023
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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