National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The long-term indiscriminate usage of antimicrobials in poultry for growth promotion and therapeutic purposes has resulted in the emergence of multi-drug resistant (MDR) bacteria like E. coli and Salmonella spp. The food-producing birds harbouring MDR genes of ESBL and Carbapenemases may possess health risks to the human population and is a major pressing issue affecting the market for broiler meat. The present study was conducted to find the occurrence of E. coli and Salmonella spp. in broiler Japanese quails of the Wayanad district and to detect the presence of ESBL and carbapenem resistance. A total of 22 cloacal swabs, two feed and two water samples were analysed for E. coli and Salmonella spp. by conventional microbiological, biochemical and molecular methods and the occurrence was observed as 100 and 9.09 per cent, respectively. The phenotypic characterization of isolates for ESBL production and carbapenem resistance was tested through disc diffusion method as per CLSI 2019 guidelines. The isolates were screened for blaCTX-M, blaSHV and blaTEM genes for ESBL genotypic characterization and 90.90 per cent of E. coli isolates were found to harbour at least one ESBL gene of interest whereas, negative results were obtained for Salmonella spp. isolates in both phenotypic and genotypic assay. None of the isolates was found positive for the presence of carbapenem-resistant S genes blaNDM, blaOXA-48, blaIMP and blaVIM. Thus, a diverse phenotypic and genotypic pattern of ESBL and carbapenem resistance was observed among isolates.
Citations |
 |
 |
 |
 |
