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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(7): 85-98
DOI: https://doi.org/10.20546/ijcmas.2022.1107.010


Molecular Characterization of Mulberry Root-Knot Nematode, Caused by Meloidogyne incognita using Modified DNA Isolation Protocol
Molecular Biology Laboratory-I, Central Sericultural Research and Training Institute,
Mysore 570 008, Karnataka, India
*Corresponding author
Abstract:

Root-knot nematodes (RKN) are notorious to cause root galls and leads to severe damage on different crops, including mulberry. RKN have very wide host range and Meloidogyne is a universal problem due to its polyphagous nature. Molecular diagnosis at species level is more efficient than the morphological identification of RKN. Hence, in this study, two methods were used for isolation of nematode DNA using root galls of mulberry. Between these, the modified CTAB extraction method yielded a high quantity of DNA (1244.8 to 7031.4 ng/µl), with spectrophotometer reading at A260 / A280 ratio ranged between 1.62 to 1.96 for purity. Further, the DNA was subjected to PCR amplification using Meloidogyne species specific markers. The best amplification was observed in DNA isolated from the modified CTAB method. The genus and species-specific markers were used for accurate detection of RKN samples collected from three mulberry growing states of South India. The amplicon size at 612 bp and 399 bp confirmed the presence of genus Meloidogyne and species M. incognita, respectively in all the samples. Further, amplified regions of 28S rDNA and SCAR were sequenced to confirm the identification of the RKN. This study revealed the association of M. incognita in mulberry root-knot nematode at the molecular level in South India.


Keywords: Root-knot nematode, Mulberry, DNA, Meloidogyne incognita, CTAB
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How to cite this article:

Haniyambadi B. Manojkumar, Arunakumar Gondi Somashekharappa and Belaghihalli N. Gnanesh. 2022. Molecular Characterization of Mulberry Root-Knot Nematode, Caused by Meloidogyne incognita using Modified DNA Isolation Protocol.Int.J.Curr.Microbiol.App.Sci. 11(7): 85-98. doi: https://doi.org/10.20546/ijcmas.2022.1107.010