'' '' '' '' Bioprospecting of Actinobacteria in Rhizosphere of Amazonic Soils and Evaluation of Biotechnological Potential for the Production of Antimicrobials - Igarapé-Açu, Pará, Brazil
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles                      Volume : 11, Issue:3, March, 2022

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(3): 201-210
DOI: https://doi.org/10.20546/ijcmas.2022.1103.024


Bioprospecting of Actinobacteria in Rhizosphere of Amazonic Soils and Evaluation of Biotechnological Potential for the Production of Antimicrobials - Igarapé-Açu, Pará, Brazil
Lívia Freitas da Silva Pinto iD1*, Daniel dos Santos Caldas iD1, Maria Clara Coelho Prazeres iD1, Juliana Hiromi Emin Uesugi iD1, Jonatan Carlos Cardoso da Silva iD1, Caroline Ferreira Fernandes iD1 and Nilson Veloso Bezerra iD2
1Biomedicine undergraduate student at Pará State University, Marco, Belém – PA, Brazil, 66807-662
2Professor at Pará State University; PhD in Agronomy (Agricultural Entomology) from Paulist State University Júlio de Mesquita Filho, UNESP, Brazil; Laboratory of Clinical Microbiology - UEPA, Marco, Belém - PA, Brazil, 66807-662
*Corresponding author
Abstract:

Actinobacteria are important components of the microbial population in most soils and are widely distributed and abundant, especially in the rhizosphere. The phylum Actinobacteria, particularly the genus Streptomyces, is recognized for its potential to produce a vast quantity of secondary metabolites, among which there are bioactive compounds used as antibiotics. The present study sought to isolate and identify actinobacteria from secondary forest rhizosphere in the region of Igarapé-Açu and to evaluate the biotechnological potential of these strains for the production of antimicrobials. Thirty rhizospheric soil samples were collected, pre-treated and seeded on Czapeck Dox and Saboraud Dextrose Agar. The methodology applied consisted of phenotypic analysis, morphological (macroscopic and microscopic) and biochemical characterization. In addition, the isolated strains were submitted to antimicrobial potential evaluation tests in order to verify if they were effective in inhibiting the growth of the multi-resistant pathogenic bacteria Escherichia coli and Klebsiella pneumoniae. We identified 18 strains of actinobacteria with the following presumptive genera: Streptomyces, Nocardia and Actinoplanes. Among the 18 strains, 10 were able to form at least a halo of inhibition against one of the pathogenic bacteria.


Keywords: Bioprospecting, Secondary metabolites, Antimicrobial activity, Bacterial resistance
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How to cite this article:

Lívia Freitas da Silva Pinto, Daniel dos Santos Caldas, Maria Clara Coelho Prazeres, Juliana Hiromi Emin Uesugi, Jonatan Carlos Cardoso da Silva, Caroline Ferreira Fernandes and Nilson Veloso Bezerra. 2022. Bioprospecting of Actinobacteria in Rhizosphere of Amazonic Soils and Evaluation of Biotechnological Potential for the Production of Antimicrobials - Igarapé-Açu, Pará, Brazil.Int.J.Curr.Microbiol.App.Sci. 11(3): 201-210. doi: https://doi.org/10.20546/ijcmas.2022.1103.024
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.