National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Anthracnose is an important fungal disease of papaya and caused major losses to its production worldwide. Common management of the disease involves the use of pesticides however, due to the increasing concern on the use of pesticides, alternative control have been encouraged. Use of microbial control agents are one of the potential alternatives to the management of the disease. Hence, this study was conducted to evaluate the bioefficacy of B. amyloliquefaciens strain DGA14as potential microbial control agent against C. gloeosporioides Penz causing papaya anthracnose under in vitro and in vivo test conditions and to determine the most effective level of B. amyloliquefaciens against C. gloeosporioides. Increasing levels of the bacterium such as 15 ml, 20 ml, 25 ml, and 30 ml per liter water were tested against the pathogen and were compared to control (sterilized distilled water). The experiments were laid out in Completely Randomized Design (CRD) with three replications. The data were statistically analyzed using the Analysis of Variance (ANOVA) and the treatment means were compared using Tukey’s Honest Significant Difference (HSD). Results in in vitro test showed that Bacillus amyloliquefaciens at 30ml per liter of water significantly inhibited C. gloeosporioides with zone of growth of 13.30mm and subsequent growth inhibition of 61.75%. Results of the in vivo test likewise showed that B. amyloliquefaciens at 30ml per liter of water applied as fruit-dip delayed the appearance of anthracnose symptom by two days and significantly reduced the severity of papaya anthracnose (DSI=1.42).
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