National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Rising antimicrobial resistance is a major threat in the management of infections caused by Pseudomonas aeruginosa and Acinetobacter baumannii. Colistin is considered as last resort in management of these infections. This study aims to determine prevalence of carbapenem resistance in P.aeruginosa and A.baumannii, to characterize the prevalent carbapenem resistance mechanisms and to evaluate their in vitro colistin susceptibility. Materials and methods: 150 consecutive (75 P.aeruginosa and 75 A.baumanni) isolates were included. Antimicrobial susceptibility testing was performed as per CLSI guidelines. Imipenem MIC was determined by E-test. Carbapenemase production tested by Modified Hodge test and MBL detection. Genotypic confirmation was done by PCR. Colistin MIC was determined by E-test. Results: Prevalence of MDR among P.aeruginosa was 29.3% and among A.baumannii was 50.7%. Among P.aeruginosa, 5.3% were carbapenemase producer by MHT and all were found to be MBL by Imipenem-EDTA combined disc method. Among A.baumannii, 12% were positive for carbapenemase production by MHT; 4% were MBL producers. 4 of 19 carbapenem resistant isolates were having MIC in upper limit of susceptible range (1.5 to 2 µg/ml). Conclusion: Because carbapenem resistance is also associated with resistance to antibiotics of other classes, the therapeutic options are very limited. MIC testing should be performed for colistin.
Citations |
 |
 |
 |
 |
