National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Astudy was designed to isolate and characterize the Shigella spp., from raw meat sold in Puducherry. A total of 150 raw meat samples (Chicken, chevon and beef each 50) were analyzed. Overall prevalence of 28(18.7%) isolates recovered and species were of Sh.dysenteriae and Sh. flexneri. Amongst 23 isolates maximum was recovered from chicken meat 14 (50%) followed by 7 (25%) chevon and 7 (25%) beef. Sh. dysenteriae was higher than Sh.flexneri. All the Sh. dysenteriae isolates were confirmed by PCR. Out of 14 PCR confirmed isolates of Sh. dysenteriae, 13 (92.8%) produced biofilm on modified congo red agar. In a total of 14 cultural and biochemical tests confirmed isolates of Sh.flexneri, 9 (64.2%) were of biofilm producers. Isolates showed maximum sensitivity against cefotaxime, azithromycin and cephalexin and complete resistance against amoxicillin and metronidazole. The high level of bacteria which could cause food borne illness in various foods presents public health risk to the consumer. This suggests the need to implement strict hygienic control measures along the food chain to improve the hygienic conditions during manufacturing, handling, storage and commercialization of foods.
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