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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(11): 170-187
DOI: https://doi.org/10.20546/ijcmas.2021.1011.020


Biocontrol of Toxin Producing Cyanobacterium Microcystis aeruginosa by Algicidal Bacterium Exiguobacterium acetylicum Strain TM2 Isolated from Mid-Altitudinal Himalayan Lake of Northern India
Surabhi Rawat1, Neetu Shahi1*, Sumanta Kumar Mallik1, Richa Pathak1, Bhupendra Singh1 and Veena Pande2
1ICAR-Directorate of Coldwater Fisheries Research, Anusandhan Bhavan, Industrial Area, Bhimtal 263136, Nainital, Uttarakhand, India
2Department of Biotechnology, Bhimtal, Kumaun University, 263136, Nainital,Uttarakhand, India
*Corresponding author
Abstract:

Microcystis aeruginosa is a hepatotoxin producing cyanobacteria, found globally in freshwaters. In the present study, an algicidal bacterium against M. aeruginosa was isolated from Bhimtal Lake (29°20’39”N; 79°33’32”E) of Himalayan region of Uttarakhand, India. The isolated bacterium Exiguobacterium acetylicum strainTM2was identified by morphological characteristics, biochemical characteristics and partial 16S ribosomal DNA (rDNA) gene amplification (GenBank accession number: KX155561). Efficacy of E. acetylicum TM2, and its mode of algicidal activity was evaluated against M. aeruginosa. E. acetylicum TM2 showed intense anti-cyanobacterial effect against M. aeruginosa, and approximately 90.0 % death of M. aeruginosa cells were observed after 10 days of incubation. The bacterium attacked the M. aeruginosa cells directly by physically coming in contact and caused damaged to its membrane and internal organelles. Cell free filtrate of E. acetylicum TM2 did not exhibited algicidal activity, which indicates that mode of algicidal mechanism, is cell to cell contact, and not chemically mediated damage by algicidal compounds released from E. acetylicum TM2. Furthermore, in vitro and in vivo pathogenicity test confirm the non-virulence of E. acetylicum TM2 and so it could be potentially useful in mitigation of M. aeruginosa blooms in water.


Keywords: Microcystis, Algicidal bacteria, Algal bloom, Biological control, Himalaya
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How to cite this article:

Surabhi Rawat, Neetu Shahi, Sumanta Kumar Mallik, Richa Pathak, Bhupendra Singh and Veena Pande. 2021. Biocontrol of Toxin Producing Cyanobacterium Microcystis aeruginosa by Algicidal Bacterium Exiguobacterium acetylicum Strain TM2 Isolated from Mid-Altitudinal Himalayan Lake of Northern India.Int.J.Curr.Microbiol.App.Sci. 10(11): 170-187. doi: https://doi.org/10.20546/ijcmas.2021.1011.020