National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was designed to evaluate Preliminary phytochemical analysis, in vitro antimicrobial activity and antioxidant activity of different six extracts of Desmostachya Bipinnata Stapf (Kush). Plant roots were extracted in different six solvents viz. Hexane, ethyl acetate, acetone, Methanol, Water and Methanol: Water (90:10) through Soxtherm according to polarity gradients. The phenolics, flavonoid, tannin and other phytochemicals of the extract were also determined using standard phytochemical reaction methods. Methanol: water and methanol extracts showed the presence of Phenol and glycosides, while in acetone extract terpenoids and glycosides were found. Aiming to investigate antimicrobial activities, agar well diffusion method was followed using three pathogenic bacteria and two fungi as test organisms. The plant root extracts showed moderate antibacterial activities (zone of inhibition (ZOI): 6-9mm) which was compared with standard kanamycin, while extracts showed positive antifungal activities (ZOI: 6-12 mm) and fluconazole was used as standard antifungal agent. We assessed the antioxidant potential of all six extracts of Desmostachya bipinnata Stapf (Kush) using test involving inhibition of DPPH activities. The highest antioxidant activity of acetone extract was noticed at IC50 (Inhibition concentration at 50%) of 17.42μg /ml followed by methanol extract at IC50 of 25.83 μg /ml compared to those of ascorbic acid (7.5 μg /ml). Current studies indicated that plant root extracts possessed moderate antimicrobial activities and good antioxidant activity. So our findings revealed that the acetone extract of Desmostachya bipinnata Stapf (Kush) possess antioxidant properties and could serve as free radical inhibitors or scavenger or, acting possibly as natural antioxidants.
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