Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(9): 284-290
DOI: https://doi.org/10.20546/ijcmas.2021.1009.033


Evaluation of Genetic Variability of Cryptococcus neoformans VNB Isolates
Luísa Silva Nangi dos Santos1, OseiYaw Nyarko2 and Leonardo Euripedes de Andrade e Silva2*
1Multiprofessional Health Residency Program,
2Department of Infectious Disease, Triangulo Mineiro Federal University, Uberaba, Brazil
*Corresponding author
Abstract:

Phylogenetic analysis of pathogenic microorganisms contributes to better understanding the distribution of genotypes that may be specific of certain regions and maybe associated with an increased virulence or antifungal resistance. This study performed phylogenetic analysis of 105 Sequence Types (ST) described for the VNB genotype of Cryptococcus neoformans available on the MLST database in order to better understand this population structure. We found three main clusters, being Cluster 1 the largest with 83 STs and Cluster 2 the smallest with three STs. In general, the isolates presented high genetic variability with haplotype diversity (HD) of 0.998 and nucleotide diversity (π)of 0.00372.Results also demonstrated recombination events, although PHY test showed no significance (p=5.5511). These findings are important since they illustrate that a genotype previously restricted to Africa and now distributed worldwide presents high genetic variability, with potential impact in the development of increased virulence, antifungal resistance, among other factors.


Keywords: Cryptococcus neoformans, MLST, Cryptococcosis, VNB genotype, phylogenetic analysis
Download this article as Download

How to cite this article:

Luísa Silva Nangi dos Santos, OseiYaw Nyarko and Leonardo Euripedes de Andrade e Silva. 2021. Evaluation of Genetic Variability of Cryptococcus neoformans VNB Isolates.Int.J.Curr.Microbiol.App.Sci. 10(9): 284-290. doi: https://doi.org/10.20546/ijcmas.2021.1009.033