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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(8): 523-530
DOI: https://doi.org/10.20546/ijcmas.2021.1008.062


Coagulase-Negative Staphylococci (CoNS) Positivity and Sensitivity in Invasive Blood Stream Infections using Automated Bactec in Tertiary care Hospital of a Hilly State in India
Pankaj Katoch1*, Ambika Soodand Surinder Singh2
1Department of Microbiology,
2Department of Paediatrics, IGMC, Shimla, India
*Corresponding author
Abstract:

Today, in the present-day scenario CoNS, as typical opportunistic infections, represent one of the significant nosocomial pathogens, having a huge impact on human health and life. It is very difficult to determine whether positivity is due to bacteremia or contamination. Hence, the present study is done to determine the Coagulase-Negative Staphylococci (CoNS) positivity in Invasive bloodstream infections using Automated Bactec systems. All the blood culture samples received in the Department of Microbiology for culture by Bactec Bd fx from July 2015 to June 2016 were included in the study. The blood culture was observed in the Bactec Bd fx system for at least 5 days before they are reported as sterile. Among the total 1275 cultures which were positive for bacteria, 344(26.98%) were of Gram-positive bacteria while 931(73.02%) were positive for Gram-negative bacteria. Among the total of 344 culture positive for Gram-positive bacteria, CoNS was isolated in 143 (41.57%) cultures. Among the total, maximum CoNS were found in the age group of 0-1 years (35.66%) followed by the 19-45 year age group (20.98%). All the Coagulase-negative Staphylococcus (CoNS) isolates showed 100% sensitivity to Vancomycin, Gentamycin, Teicoplanin, and Linezolid. Besides, that 91.67% of isolates were sensitive to Levofloxacin, 87.5% Tetracycline, 80%Aztreonam, and 72% to Piperacillin. It was found that Coagulase-negative Staphylococcus (CoNS) showed high-level resistance to Penicillin (53.85%), Ceftazidime (52.00%) Clindamycin (47.01%), and Cotrimoxazole (41.96%). CoNS was one of the most common organisms among GPC isolates and the commonest isolated in the neonate and infant age group. All the CoNS isolates showed maximum sensitivity to Vancomycin, Gentamycin, Teicoplanin, and Linezolid while it showed high-level resistance to Penicillin, Ceftazidime, Clindamycin, and Cotrimoxazole. Strict aseptic procedures to be implemented during patient's stay in the hospital to minimize nosocomial infections.


Keywords: Blood Stream Infections, Culture, Bactec, Positivity, Sensitivity, Coagulase-Negative Staphylococci (CoNS)
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How to cite this article:

Pankaj Katoch, Ambika Sood and Surinder Singh. 2021. Coagulase-Negative Staphylococci (CoNS) Positivity and Sensitivity in Invasive Blood Stream Infections using Automated Bactec in Tertiary care Hospital of a Hilly State in India.Int.J.Curr.Microbiol.App.Sci. 10(8): 523-530. doi: https://doi.org/10.20546/ijcmas.2021.1008.062