National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Total of 523 bacterial isolates were collected from studied children groups (7-10 years); caries-active (n=50), caries-free (n=50). From caries-active children, 325 isolates have belonged to 22 bacterial species, whereas, in caries-free children, 198 bacterial isolates have belonged to 18 species. All isolated bacterial species were fit in 6 different bacterial genera included Actinomyces, Lactobacillus, Porphyromonas, Prevotella, Streptococcus and Veillonella. The predominant bacteria were Streptococcus mutans(96%) and Str. orali(56%) in caries-active children. In caries-free children, the most frequently detected species included Lactobacillus acidophilus (72%) and Str. oralis (36%). Partial sequence of the 16S rRNA gene was determined for confirming the identification of the 22 strong-biofilm bacterial isolates. The minimum inhibitory concentration (MIC90) of Selenium nanoparticles (SeNPs) was 25 µg/mL against most of strong-biofilm bacteria. Sub-MIC90 (25 µg/mL) of SeNPs were incorporated in Poly lactic-co-glycolic acid (PLGA) to fabricate coating surface. PLGA/SeNPs coating surface showed potential reduction effect against biofilm formation by the 22 strong-biofilm strains. In conclusion, SeNPs can be used as a promising agent for effectively preventing biofilm formation by strong-biofilm bacteria related to dental caries.
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