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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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Original Research Articles                      Volume : 10, Issue:8, August, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(8): 329-333
DOI: https://doi.org/10.20546/ijcmas.2021.1008.039


Next Generation Sequencing: Principle and Applications
Arul Pandiyan* and Mahesh Dahake
Department of Veterinary Pathology, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur-440006, India
*Corresponding author
Abstract:

Next Generation Sequencing (NGS) also named as massively parallel sequencing, is a powerful new tool that can be used for the complex diagnosis and intensive monitoring of infectious diseases in veterinary medicine. NGS technologies are also being increasingly used to study the aetiology, genomics, evolution and epidemiology of infectious disease, as well as host pathogen interaction, host immune response including responses to antimicrobial treatment and vaccination. NGS approaches can be used as primary tools for the study of disease outbreaks by the identification and follow-up of transmission routes, thereby helping in the identification of outbreak origins in zoonotic diseases. The above applications of next generation sequencing in disease and cancer diagnosis, genome characterization and viral diversity, viral metagenomics and host pathogen interaction study make it a powerful tool for detailed analysis of complex populations in a minimalistic period of time.


Keywords: Next Generation Sequencing, Clinical diagnostics, Sanger sequencing, Metagenomics

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How to cite this article:

Arul Pandiyan and Mahesh Dahake. 2021. Next Generation Sequencing: Principle and Applications.Int.J.Curr.Microbiol.App.Sci. 10(8): 329-333. doi: https://doi.org/10.20546/ijcmas.2021.1008.039
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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