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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(7): 642-654
DOI: https://doi.org/10.20546/ijcmas.2021.1007.070


Biological Control of Helminthosporium mays Responsible of Maize
(Zea mays L.) Helminthosporiosis through the Application of Essential Oils of Eucalyptus citriodora and Ocimum gratissimum in Côte d'Ivoire
Coulibaly Klotioloma1*, Ouattara Adama2, Soro Sibirina3, Gogbe Françoise1,
N’guessan Walet Pierre1, Acka Kotaix1, Kouame Norbert1, Tahi Mathias1, Guiraud Brigitte1, Assi Maryse1, Kone Daouda4, Konate Ibrahim2 and N’guessan François1
1Centre National de Recherche Agronomique (CNRA), B.P 808, Divo, Côte d’Ivoire
2Laboratoire d’Agrovalorisation, Département de Biochimie-Microbiologie, UFR Agroforesterie, Université Jean Lorougnon Guédé (UJLoG), BP 150 Daloa, Côte d’Ivoire   
3Laboratoire de Physiologie et Amélioration des Productions Végétales; UFR Agroforesterie, Université Jean Lorougnon Guédé (UJLoG), BP 150 Daloa, Côte d’Ivoire
4Laboratoire de Pathologie et de Physiologie végétale, UFR Biosciences, Université Félix Houphouët-Boigny, 22 BP 582 Abidjan 22, Côte d’Ivoire
*Corresponding author
Abstract:

To control helminthosporiosis of maize (Zea mays L.) in Côte d'Ivoire, farmers mainly use chemical fungicides. However, these products are very expensive and polluting. This study aimed to propose a biocontrol strategy against the fungal agent Helminthosporium mays using essential oils of two aromatic plants (Eucalyptus citriodora and Ocimum gratissimum). Strains of Helminthosporium mays were isolated from symptomatic leaves of maize. Two maize cultivars (Cultivar jaune de Anyama CJA and Cultivar jaune de Ferké CJF) were inoculated with these fungal strains in a complete randomised Fischer block design with two replicates. The effect of essential oils on the growth of the fungal strains was tested in vitro at different concentrations (500 ppm, 1000 ppm, 3000 ppm and 5000 ppm). A total of six (06) strains of the species Helminthosporium mays were isolated. Two strains SBA and SBI from Bassam and Bingerville were the most virulent. They induced a high mean number of symptoms on the two maize cultivars respectively 25.33 and 26.13 necrotic spots per leaf. The essential oil of the species Ocimum gratissimum was the most effective as it totally inhibited mycelial growth from a concentration of 1000 ppm. This study showed the potential fungicidal effect of essential oils on this phytopathogenic fungal agent.


Keywords: Biocontrol, helminthosporiosis, essential oils, Eucalyptus citriodora, Ocimum gratissimum
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How to cite this article:

Coulibaly Klotioloma, Ouattara Adama, Soro Sibirina, Gogbe Françoise, N’guessan Walet Pierre, Acka Kotaix, Kouame Norbert, Tahi Mathias, Guiraud Brigitte, Assi Maryse, Kone Daouda, Konate Ibrahim and N’guessan François. 2021. Biological Control of Helminthosporium mays Responsible of Maize (Zea mays L.) Helminthosporiosis through the Application of Essential Oils of Eucalyptus citriodora and Ocimum gratissimum in Côte d'Ivoire.Int.J.Curr.Microbiol.App.Sci. 10(7): 642-654. doi: https://doi.org/10.20546/ijcmas.2021.1007.070