National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Phytase (myo-inositol hexakisphosphate phosphohydrolase)enzyme was isolated from wheat varieties to do kinetic study of phytate degradation. The four wheat varieties selected on the basis of their higher phytase activity for investigation were DBW-17, HD-2894, HUW-234 and LOK-1 wheat variety. Among the four selected wheat varieties, LOK -1 variety reported the maximum phytase activity during enzyme kinetics study. Partially purified phytase had pH optima at pH 5.0 with temperature optima at 60ºC, which showed maximal phytate degrading activity. The enzyme was found to have good substrate specificity for sodium phytate at 3mM and calcium phytate at 2mM concentration. Wheat phytase can be used in hydrolysis of phytate in food industries for releasing micronutrients and enhance increasing their bioavailability to chemical industry as well.
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