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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(7): 371-377
DOI: https://doi.org/10.20546/ijcmas.2021.1007.040


Kinetic study of Phytase in Four Indian Wheat Varieties  (Triticum aestivum L.)
Manish Kumar1,2* and Sushma2
1Department of Biochemistry, Institute of Life Science, Bundelkhand University,
 Jhansi-284128, India
2Department of Biochemistry and Biochemical Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj-211007, India
*Corresponding author
Abstract:

Phytase (myo-inositol hexakisphosphate phosphohydrolase)enzyme was isolated from wheat varieties to do kinetic study of phytate degradation. The four wheat varieties selected on the basis of their higher phytase activity for investigation were DBW-17, HD-2894, HUW-234 and LOK-1 wheat variety. Among the four selected wheat varieties, LOK -1 variety reported the maximum phytase activity during enzyme kinetics study. Partially purified phytase had pH optima at pH 5.0 with temperature optima at 60ºC, which showed maximal phytate degrading activity. The enzyme was found to have good substrate specificity for sodium phytate at 3mM and calcium phytate at 2mM concentration. Wheat phytase can be used in hydrolysis of phytate in food industries for releasing micronutrients and enhance increasing their bioavailability to chemical industry as well.


Keywords: Phytase, phytate, wheat, sodium and calcium phytate
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How to cite this article:

Manish Kumar and Sushma. 2021. Kinetic study of Phytase in Four Indian Wheat Varieties (Triticum aestivum L.).Int.J.Curr.Microbiol.App.Sci. 10(7): 371-377. doi: https://doi.org/10.20546/ijcmas.2021.1007.040