National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Canine Mammary Tumour (CMT) is the most common malignancy of unspayed female dogs and is of potential importance as a model for human breast cancer. Mortality rate in female dogs is three times than that of human females with breast cancer. Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) also called as CD66a (Cluster of Differentiation 66a), is a glycoprotein which is over expressed in various human cancers. Although several studies have shown correlation of gene expression with tumour progression, metastasis and overall survival in human breast cancer, very few studies have focussed so far on CEAM 1 gene and its expression in canine mammary cancers. Therefore, present study was undertaken to determine CEA-CAM1 gene sequence in a clinical case of canine mammary tumour (CMT), showing over expression of the gene and showing presence of anti-CEA autoantibodies in the serum. C-terminal immunodominant region of CEA-CAM1 gene(from case of canine mammary tumour) was amplified and cloned in prokaryotic expression vector. Further the gene was also sequenced. Alignment of CEA-CAM1 mRNA sequence from CMT tissue and normal healthy dog tissue showed 100% similarity between them showing absence of any mutation in CEA-CAM1 coding region in case of CMT suggesting that mechanisms other than gene alterations and mutations are responsible for over expression. Some possible mechanisms for production of autoantibodies against CEA-CAM1 could be due to increased antigenic load due to its over expression in tumour tissues and reduced degradation of protein etc. Phylogenetic analysis of the dog CEA-CAM1 gene showed that dog gene sequence has more than 88% nucleotide sequence identity with Mustela putorius (European polecat), Ursus maritimus (polar bear), Callorhinus ursinus (Northern fur seal) and Pantherapardus (leopard), whereas canine CEA-CAM1 sequence is 74.5% identical with Homo sapiens gene.
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