National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Aquaculture products can harbor pathogenic bacteria which are part of the natural microflora of the environment. Feeding infected fish with antibiotic- medicated food is a general practice but has led to antibiotic resistance development in bacterial pathogen, resulting in a higher dose requirement for effective control, a matter of increasing public concern. Resistance of pathogens to antibiotics occurs due to the presence of β-lactamase enzyme, thereby resulting in Multiple Drug Resistance (MDR). These enzymes are present in Enterobacteriaceae such as E. coli. This study was conducted aiming at the isolation of E. coli from the intestines, muscle, head and tail of apparently healthy freshwater fish Cirrhinus cirrhosis collected from the local dam in Coimbatore district, Tamilnadu. Differentiation and characterization of E. coli isolates was based on their growth characteristics on specific culture media. E. coli was observed to be present in higher amounts in all parts of the fish. E. coli was identified and PCR amplification was performed for the isolated E.coli. The Hi Comb assay was also performed.
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