National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Salmonellosis is one of the most common causal food borne disease in India. An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella spp. isolates in foods of animal origin from Mumbai, India. The aim of this study was to develop in-house LAMP for simple and inexpensive detection of Salmonella spp. in animal origin foods using specifically designed primers targeting invA gene contains sequences unique to this genus. The reaction was optimized using genomic DNA of S. typhimurium (MTCC 3224) as the template. The assay was conducted in a water bath for 1h at 65°C. The results were visualized after addition of SYBR Green® fluorescent dye. The test was further evaluated on 59 serotyped Salmonella field isolates to ensure its reliability and usefulness. The results were compared with those obtained by gold standard culture method and Polymerase Chain Reaction (PCR). This method was highly specific and 10 times more sensitive in detecting Salmonella spp. compared to the optimized conventional polymerase chain reaction (PCR) method. This simple method may be applied on field diagnostic laboratories without access to expensive equipment.
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