National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Internal Transcribed Spacer1 (ITS-1) of nuclear ribosomal DNA (rDNA) sequences of Trypanosoma evansi isolates from camel dromedary of King of Saudi Arabia (KSA) used to investigate evolutionary origins and infer phylogenetic relationships with other Global isolates. The study was actually started with microscopic surveillance of blood samples on 454 from naturally infected camels in Jazan region, south western, by using wet and thick smear film which revealed 3.1% (14 positive) and 3.5% (16 positive), respectively, however molecular examination was performed on 100 blood samples using conventional PCR- ITS1 targeting 480 bp and resulted 19% positive, the ITS1 product was subjected to RoTat 1.2 VSG primer (151 bp) that derived from a trypanosome specific repetitive nucleotide sequence fragment and yielded 36.8% positive. The molecular characterization and phylogenetic analysis were achieved on product sequences and aligned against the corresponding GenBank sequences of known isolates of T. evansi. Basic Local Alignment Search Tool (BLAST) data of the obtained ITS1 gene sequences showed identity to those of T. evansi, with the homology of 92% to 99%. Sequence analysis of this gene generated six heterogeneous genotypes of T. evansi in KSA are present, released in GenBank and encoded in following terms of jaz1, jaz2, jaz3, jaz4, jaz5 and jaz6 with accession numbers; MN611173, MN625863, MN625864, MN611174, MN625865 and MN625866, respectively. T. evansi isolates from KSA and from other countries were tightly clustered into the constructed phylogenetic tree based on the ITS-1 rDNA sequence using Maximum Composite Likelihood and neighbour-joining method, revealing apparently genetic diversity among the isolates. This isthe first study about sequence analysis of T. evansi which prove genetic diversity within isolates from Saudi Arabia.
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