National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Tabanidae are true flies and members of the insect order Diptera, causing serious nuisance to livestock, which leads to considerable production losses and mechanically transmit several signiï¬cant animal pathogens. In lack of data of classification and/or identification of tabanids (horse flies), additional to their seasonal abundance in the kingdom of Saudi Arabia (KSA), the present study was carried out to describe the morphological and molecular characterization of tabanid flies with density variation related. Four times a month, from October 2017 to September 2018,a total number of 457 flies’ samples were collected from Jazan region southwestern Saudi Arabia, using Nzi traps. More tabanids flies were captured during the wet 67.61% (309/457) compared to the dry season 32.39% (148/457). Four different species of three Tabanids’ genera were identified morphologically using the standard taxonomic keys; two for the first time namely; Hybomitra lurida and Tabanus par, beside Atylotus agrestis and Tabanus Sufis that were reported before in KSA. Genomic DNA was extracted from Saudi Arabian tabanids, and polymerase chain reaction (PCR) successfully amplified 710 base pairs of mitochondrial cytochrome c oxidase subunit 1 (COI) genes. Sequence analysis was performed, and three out of four species were identified (A. agrestisMW243943, H. lurida MW265638 and T. parMW238412).The product sequences of KSA tabanid flies were aligned each separately against the corresponding GenBank sequences of known specimens and showed that the specimens of A. agrestis and T. par from Saudi Arabia were closely related to that of the Afrotropical region than to the Palaearctic region, whilst the sp. H. lurida was more similar to that of the Nearctic regions. This is the first report to investigate genetic characterization and phylogenesis of tabanid flies circulating in KSA.
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