National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
High mortality rates are often associated with antimicrobial resistance, early detection of these resistant strains and their phenotypic and genotypic confirmation is necessary to decide the suitable therapy. To screen beta-lactamase production and its genes such as bla-VIM in Ps. aeruginosa strains isolated from hospitalized patients in Tanta University Hospitals. The study was carried out on 160 Pseudomonas aeruginosa isolates. Samples had been cultured, identified and tested for sensitivity. A phenotypic test (Double disc synergy test) was applied on the collected MDR isolates for detection of MBL enzyme production. Conventional PCR was performed for the detection of the resistance gene(bla-VIM). 100 isolates were multi-drug resistant according to (Modified Kirby Bauer disc diffusion method), with sensitivity to some drugs specially Amikacin (20%). Phenotypic test for MBL resulted in (38%) positive isolates and (62%) were negative ones. Multiplex PCR detected gene (bla-VIM) in (30%) isolates and it was absent in (70%) of the isolates. High prevalence of MBL among multi-drug resistant P. aeruginosa is thought to be due to the uncontrolled consumption of antimicrobials. Rapid identification of such strains is needed to decrease mortalities.
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