National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An investigation was carried out to screen the efficient urban solid waste degrading microorganisms for the formulation of microbial consortium. During the process of degradation, bacterial population was dominated over fungal and actinobacteria. The succession of microorganisms during degradation process showed bacterial population (58×106 cfu/g) was found highest compared to fungal population (39×103 cfu/g) and actinobacterial (24x103cfu/g) population. Totally fifty five bacterial, thirty two fungal and seven actinobacterial strains were isolated during sixty days of urban solid waste incubation. All the bacterial isolates were found rod shaped and non-endospore formers. Among the bacterial isolates, UWCB-16, UWCB-29 and UWCB-34 were Gram positive. All the isolated organisms showed positive for the cellulase, pectinase, protease, amylase, ligninase and chitinase production. Fungal isolate UWCF-07 (27 mm) showed highest zone followed by the bacterial isolate UWCB-25 (26 mm) for cellulase production. Bacterial isolate UWCB-34 (26 mm) recorded highest pectinase producing ability followed by fungal isolate UWCF-07 (24 mm), where as protease and amylase production was found in the bacterial isolates UWCB-34 (23 mm) and UWCB-25 (20 mm) respectively. Ligninase production ability was highest in fungal isolates. Fungal isolate UWCF-09 and 07 (107.91 and 106.48) showed significant reduction in the C: N followed by actinobacterial isolate UWCA-05 (111.72) and bacterial isolate UWCB-25 (114.18) during decomposition of saw dust with urban solid waste. Among twelve microbial cultures screened, six cultures were selected to form the consortium based on their ability to produce enzymes, carbon and nitrogen mineralization efficiency, which includes three fungi (Trichoderma harzianum (UWCF-09), Penicillium polonicum (UWCF-07) and Coprinus sp. (UWCF-02)), two bacteria (Bacillus cereus (UWCB-34), Bacillus licheniformis (UWCB-25)) and an actinobacteria (Streptomyces albus (UWCA-05)) respectively.
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