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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 1879-1891
DOI: https://doi.org/10.20546/ijcmas.2021.1003.237


Characterization of Transgenic Cotton (Gossypium hirsutum L.) Lines against Moisture Stress Carrying AtDREB1A Gene
Dattatraya Hegde Radhika1*, Ishwarappa S. Katageri1, H. M. Vamadevaiah2,Shruthi Kamplikoppa2 and Vishal Kumar1
1Institute of Agri-Biotechnology, Department of Biotechnology, University of Agricultural Sciences, Dharwad, Karnataka, India
2Agriculture Research Station Hebballi, University of Agricultural Sciences, Dharwad, India
*Corresponding author
Abstract:

Drought tolerance is accompanied by number of traits and they are regulated by the many genes, hence targeting single gene against moisture stress will not be effective in crop improvement. Use of transcription factors (TFs) to target corresponding multigene is current trend. As per the many research findings, dehydration responsive element binding proteins (DREB) are reported as an important TFs that known to induces number of abiotic stress-related genes and impart the stress tolerance in plants. With this perspective, cotton variety Coker-312 was introduced with AtDREB1A gene and in the present study, transgenic cotton lines of T2 generation were evaluated for moisture stress resistance at Agriculture Research Station, Dharwad. Segregating T2 lines were screened with the help of PCR using gene specific primer and during moisture stress, DREB1A gene and its target gene fold change was studied using real time PCR (RT-PCR). Higher accumulation of the osmo-protectants like proline, reducing sugar was observed in the transgenic lines as compared to the non-transgenic lines under moisture stress.


Keywords: AtDREB1A, Cotton, Fiber, LEA protein gene, PCR, Proline, Reducing sugar, RT-PCR, Transgenic
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How to cite this article:

Dattatraya Hegde Radhika, Ishwarappa S. Katageri, H. M. Vamadevaiah, Shruthi Kamplikoppa and Vishal Kumar. 2021. Characterization of Transgenic Cotton (Gossypium hirsutum L.) Lines against Moisture Stress Carrying AtDREB1A Gene.Int.J.Curr.Microbiol.App.Sci. 10(3): 1879-1891. doi: https://doi.org/10.20546/ijcmas.2021.1003.237